Effect of n-3 rich fish oil supplementation on the mechanisms related to lymphocyte differentiation in overweight and obese woman

• Grant number: 16/25425-2
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): March 01, 2017
• Effective date (End): December 31, 2017
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: Renata Gorjao
• Grantee: Richelieau Manoel
• Home Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil

Abstract

Studies have demonstrated an increase of overweight and obesity in the last years. This fact is highlighted in elderly population in which obesity increased significantly. Aging and obesity are related to several disorders modifying the immune homeostasis. These alterations are due to chronic inflammation. Recent studies have demonstrated that n-3 fatty acid supplementation have beneficial effects on inflammatory diseases and other disturbs related to obesity. Therefore, the aim of this study is to evaluate the effect of n-3 rich fish oil supplementation on expression of genes related to lymphocyte differentiation in woman above 60 years of age. Thirty overweight or obese women will be recruited from São Miguel Paulista neighborhood in the São Miguel Paulista Sports Center from Cruzeiro do Sul University. The evaluations will be performed in two moments: before and after three months of fish oil or soy lecithin supplementation (control group). Anthropometric and body composition will be evaluated by weigh, height, body circumferences and fat percentage by bioimpedance. IL-35, TGF-beta, Foxp3, Tbet, ROR±, RORgt, GATA3, STAT1, 3 and 4 and Blimp-1 mRNA expression will be analyzed by real time PCR. The percentage of Th1, Th2, Th17 and T regulatory cells will be evaluated by flow cytometry. The modulation of molecular mechanisms involved with lymphocyte differentiation by n-3 fatty acids will be elucidated in the present study leading to a better application of these fatty acids in elderly population. (AU)