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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Behavioral and genetic effects promoted by sleep deprivation in rats submitted to pilocarpine-induced status epilepticus

Texto completo
Autor(es):
Matos, Gabriela [1] ; Ribeiro, Daniel A. [2] ; Alvarenga, Tathiana A. [1] ; Hirotsu, Camila [1] ; Scorza, Fulvio A. [3] ; Le Sueur-Maluf, Luciana [2] ; Noguti, Juliana [4] ; Cavalheiro, Esper A. [3] ; Tufik, Sergio [1] ; Andersen, Monica L. [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, BR-04024002 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Biociencias, BR-04024002 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Neurol Expt, BR-04024002 Sao Paulo - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Dept Patol, BR-04024002 Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Neuroscience Letters; v. 515, n. 2, p. 137-140, MAY 2 2012.
Citações Web of Science: 8
Resumo

The interaction between sleep deprivation and epilepsy has been well described in electrophysiological studies, but the mechanisms underlying this association remain unclear. The present study evaluated the effects of sleep deprivation on locomotor activity and genetic damage in the brains of rats treated with saline or pilocarpine-induced status epilepticus (SE). After 50 days of pilocarpine or saline treatment, both groups were assigned randomly to total sleep deprivation (TSD) for 6 h, paradoxical sleep deprivation (PSD) for 24 h, or be kept in their home cages. Locomotor activity was assessed with the open field test followed by resection of brain for quantification of genetic damage by the single cell gel electrophoresis (comet) assay. Status epilepticus induced significant hyperactivity in the open field test and caused genetic damage in the brain. Sleep deprivation procedures (TSD and PSD) did not affect locomotor activity in epileptic or healthy rats, but resulted in significant DNA damage in brain cells. Although PSD had this effect in both vehicle and epileptic groups. TSD caused DNA damage only in epileptic rats. In conclusion, our results revealed that, despite a hack of behavioral effects of sleep deprivation. TSD and PSD induced genetic damage in rats submitted to pilocarpine-induced SE. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)

Processo FAPESP: 98/14303-3 - Center for Sleep Studies
Beneficiário:Sergio Tufik
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 10/15110-8 - Sono e morte súbita nas epilepsias: provável base molecular?
Beneficiário:Gabriela de Matos Barbosa Pimenta
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 09/01030-5 - Efeito da privação e restrição de sono na função reprodutiva de ratos machos
Beneficiário:Tathiana Aparecida Fernandes de Alvarenga
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 10/50129-1 - Insuficiência renal crônica, restrição de sono e sildenafil: consequências renais, cardíacas e sexuais em um modelo animal
Beneficiário:Camila Hirotsu
Linha de fomento: Bolsas no Brasil - Doutorado Direto