Lower numbers of natural killer T cells in HIV-1 and Mycobacterium leprae co-infected patients

Autor(es)

Carvalho, Karina I. ; Bruno, Fernanda R. ; Snyder-Cappione, Jennifer E. ^[1] ; Maeda, Solange M. ^[2] ; Tomimori, Jane ^[2] ; Xavier, Marilia B. ^[3] ; Haslett, Patrick A. ^[4] ; Nixon, Douglas F. ^[1] ; Kallas, Esper G. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Calif San Francisco, San Francisco Gen Hosp, Dept Med, Div Expt Med, San Francisco, CA - USA

^[2] Univ Fed Sao Paulo, Sao Paulo - Brazil

^[3] Fed Univ Para, BR-66059 Belem, Para - Brazil

^[4] Shire Human Genet Therapies, Lexington, MA - USA

^[5] Univ Sao Paulo, Lab Invest Med 60, Div Clin Immunol & Allergy, BR-01246903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: IMMUNOLOGY; v. 136, n. 1, p. 96-102, MAY 2012.
• Citações Web of Science: 3

Resumo

Natural killer T (NKT) cells are a heterogeneous population of lymphocytes that recognize antigens presented by CD1d and have attracted attention because of their potential role linking innate and adaptive immune responses. Peripheral NKT cells display a memory-activated phenotype and can rapidly secrete large amounts of pro-inflammatory cytokines upon antigenic activation. In this study, we evaluated NKT cells in the context of patients co-infected with HIV-1 and Mycobacterium leprae. The volunteers were enrolled into four groups: 22 healthy controls, 23 HIV-1-infected patients, 20 patients with leprosy and 17 patients with leprosy and HIV-1-infection. Flow cytometry and ELISPOT assays were performed on peripheral blood mononuclear cells. We demonstrated that patients co-infected with HIV-1 and M.leprae have significantly lower NKT cell frequencies {[}median 0.022%, interquartile range (IQR): 0.0070.051] in the peripheral blood when compared with healthy subjects (median 0.077%, IQR: 0.0320.405, P < 0.01) or HIV-1 mono-infected patients (median 0.072%, IQR: 0.0300.160, P < 0.05). Also, more NKT cells from co-infected patients secreted interferon-? after stimulation with DimerX, when compared with leprosy mono-infected patients (P = 0.05). These results suggest that NKT cells are decreased in frequency in HIV-1 and M.leprae co-infected patients compared with HIV-1 mono-infected patients alone, but are at a more activated state. Innate immunity in human subjects is strongly influenced by their spectrum of chronic infections, and in HIV-1-infected subjects, a concurrent mycobacterial infection probably hyper-activates and lowers circulating NKT cell numbers. (AU)

• Processo FAPESP: 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/05845-0 - Resposta imune celular em doenças infecciosas e imunodeficiências primárias
• Beneficiário: Esper Georges Kallás
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional