| Full text | |
| Author(s): |
Carvalho, Karina I.
;
Bruno, Fernanda R.
;
Snyder-Cappione, Jennifer E.
[1]
;
Maeda, Solange M.
[2]
;
Tomimori, Jane
[2]
;
Xavier, Marilia B.
[3]
;
Haslett, Patrick A.
[4]
;
Nixon, Douglas F.
[1]
;
Kallas, Esper G.
[5]
Total Authors: 9
|
| Affiliation: | [1] Univ Calif San Francisco, San Francisco Gen Hosp, Dept Med, Div Expt Med, San Francisco, CA - USA
[2] Univ Fed Sao Paulo, Sao Paulo - Brazil
[3] Fed Univ Para, BR-66059 Belem, Para - Brazil
[4] Shire Human Genet Therapies, Lexington, MA - USA
[5] Univ Sao Paulo, Lab Invest Med 60, Div Clin Immunol & Allergy, BR-01246903 Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | IMMUNOLOGY; v. 136, n. 1, p. 96-102, MAY 2012. |
| Web of Science Citations: | 3 |
| Abstract | |
Natural killer T (NKT) cells are a heterogeneous population of lymphocytes that recognize antigens presented by CD1d and have attracted attention because of their potential role linking innate and adaptive immune responses. Peripheral NKT cells display a memory-activated phenotype and can rapidly secrete large amounts of pro-inflammatory cytokines upon antigenic activation. In this study, we evaluated NKT cells in the context of patients co-infected with HIV-1 and Mycobacterium leprae. The volunteers were enrolled into four groups: 22 healthy controls, 23 HIV-1-infected patients, 20 patients with leprosy and 17 patients with leprosy and HIV-1-infection. Flow cytometry and ELISPOT assays were performed on peripheral blood mononuclear cells. We demonstrated that patients co-infected with HIV-1 and M.leprae have significantly lower NKT cell frequencies {[}median 0.022%, interquartile range (IQR): 0.0070.051] in the peripheral blood when compared with healthy subjects (median 0.077%, IQR: 0.0320.405, P < 0.01) or HIV-1 mono-infected patients (median 0.072%, IQR: 0.0300.160, P < 0.05). Also, more NKT cells from co-infected patients secreted interferon-? after stimulation with DimerX, when compared with leprosy mono-infected patients (P = 0.05). These results suggest that NKT cells are decreased in frequency in HIV-1 and M.leprae co-infected patients compared with HIV-1 mono-infected patients alone, but are at a more activated state. Innate immunity in human subjects is strongly influenced by their spectrum of chronic infections, and in HIV-1-infected subjects, a concurrent mycobacterial infection probably hyper-activates and lowers circulating NKT cell numbers. (AU) | |
| FAPESP's process: | 04/15856-9 - Prospective analysis of the virological and immunological characteristics in individuals with recent HIV-1 infection in the cities of São Paulo and Santos |
| Grantee: | Ricardo Sobhie Diaz |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 10/05845-0 - Cellular immune responses in infectious diseases and primary immunodeficiencies |
| Grantee: | Esper Georges Kallás |
| Support Opportunities: | Research Grants - Visiting Researcher Grant - International |