SUSCEPTIBILITY OF PERITONEAL MACROPHAGE FROM DIFFERENT SPECIES OF NEOTROPICAL PRIMATES TO EX VIVO Leishmania (L.) infantum chagasi-INFECTION

Autor(es)

Carneiro, Liliane Almeida ^[1] ; Laurenti, Marcia Dalastra ^[2] ; Campos, Marliane Batista ^[1] ; de Castro Gomes, Claudia Maria ^[2] ; Pereira Corbett, Carlos Eduardo ^[2] ; Silveira, Fernando Tobias ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Minist Hlth, Evandro Chagas Inst, Belem, Para - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil

^[3] Fed Univ Para, Inst Trop Med, BR-66059 Belem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 54, n. 2, p. 95-101, MAR-APR 2012.
• Citações Web of Science: 4

Resumo

This study examined the susceptibility of peritoneal macrophage (PM) from the Neotropical primates: Callithrix jacchus, Callithrix penicillata, Saimiri sciureus, Aotus azarae infulatus and Callimico goeldii to ex vivo Leishmania (L.) infantum chagasi-infection, the etiological agent of American visceral leishmaniasis (AVL), as a screening assay for evaluating the potential of these non-human primates as experimental models for studying AVL. The PM-susceptibility to infection was accessed by the PM-infection index (PMI) at 24, 72 h and by the mean of these rates (FPMI), as well as by the TNF-alpha, IL-12 (Capture ELISA) and Nitric oxide (NO) responses (Griess method). At 24h, the PMI of A. azarae infulatus (128) was higher than those of C. penicillata (83), C. goeldii (78), S. sciureus (77) and C. jacchus (55). At 72h, there was a significant PMI decrease in four monkeys: A. azarae infulatus (128/37), C. penicillata (83/38), S. sciureus (77/38) and C. jacchus (55/12), with exception of C. goeldii (78/54). The FPMI of A. azarae infulatus (82.5) and C. goeldii (66) were higher than C. jacchus (33.5), but not higher than those of C. penicillata (60.5) and S. sciureus (57.5). The TNF-alpha response was more regular in those four primates which decreased their PMI at 24/72 h: C. jacchus (145/122 pg/mu L), C. penicillata (154/130 pg/mu L), S. sciureus (164/104 pg/mu L) and A. azarae infulatus (154/104 pg/mu L), with exception of C. goeldii (38/83 pg/mu L). The IL-12 response was mainly prominent in A. infulatus and C. goeldii which presented the highest FPMI and, the NO response was higher in C. goeldii, mainly at 72 h. These findings strongly suggest that these New World primates have developed a resistant innate immune response mechanism capable of controlling the macrophage intracellular growth of L. (L.) i. chagasi-infection, which do not encourage their use as animal model for studying AVL. (AU)

• Processo FAPESP: 06/56319-1 - Leishmanioses no Brasil: aspectos clínicos e imunopatogenéticos da doença humana e experimental
• Beneficiário: Carlos Eduardo Pereira Corbett
• Modalidade de apoio: Auxílio à Pesquisa - Temático