Creatine Supplementation Prevents the Accumulation of Fat in the Livers of Rats Fed a High-Fat Diet

Autor(es):Mostrar menos -

Deminice, Rafael ^{[1, 2]} ; da Silva, Robin P. ^[1] ; Lamarre, Simon G. ^[1] ; Brown, Colin ; Furey, George N. ^[1] ; McCarter, Shannon A. ^[1] ; Jordao, Alceu Afonso ^[2] ; Kelly, Karen B. ^{[3, 4]} ; King-Jones, Kirst ^[5] ; Jacobs, Rene L. ^{[3, 4]} ; Brosnan, Margaret E. ^[1] ; Brosnan, John T. ^[1]

Afiliação do(s) autor(es)

^[1] Mem Univ Newfoundland, Dept Biochem, St John, NF - Canada

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Lab Nutr & Metab, Sao Paulo - Brazil

^[3] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB - Canada

^[4] Univ Alberta, Grp Mol & Cell Biol Lipids, Edmonton, AB - Canada

^[5] Univ Alberta, Dept Biol Sci, Edmonton, AB - Canada

• Tipo de documento: Artigo Científico
• Fonte: Journal of Nutrition; v. 141, n. 10, p. 1799-1804, OCT 2011.
• Citações Web of Science: 36

Resumo

The aim of the present study was to examine the effects of creatine supplementation on liver fat accumulation induced by a high-fat diet in rats. Rats were fed 1 of 3 different diets for 3 wk: a control liquid diet (C), a high-fat liquid diet (HF), or a high-fat liquid diet supplemented with creatine (HFC). The C and HF diets contained, respectively, 35 and 71% of energy derived from fat. Creatine supplementation involved the addition of 1% (wt:v) of creatine monohydrate to the liquid diet. The HF diet increased total liver fat concentration, liver TG, and liver TBARS and decreased the hepatic S-adenosylmethionine (SAM) concentration. Creatine supplementation normalized all of these perturbations. Creatine supplementation significantly decreased the renal activity of L-arginine:glycine amidinotransferase and plasma guanidinoacetate and prevented the decrease in hepatic SAM concentration in rats fed the HF diet. However, there was no change in either the phosphatidylcholine:phosphatidylethanolamine (PE) ratio or PE N-methyltransferase activity. The HF diet decreased mRNA for PPAR as well as 2 of its targets, carnitine palmitoyltransferase and long-chain acylCoA dehydrogenase. Creatine supplementation normalized these mRNA levels. In conclusion, creatine supplementation prevented the fatty liver induced by feeding rats a HF diet, probably by normalization of the expression of key genes of beta-oxidation. J. Nutr. 141: 1799-1804, 2011. (AU)

• Processo FAPESP: 07/08099-5 - Efeitos da suplementação de creatina sobre homocisteína e estresse oxidativo após exercício agudo aeróbio e anaeróbio em ratos
• Beneficiário: Rafael Deminice
• Linha de fomento: Bolsas no Brasil - Doutorado