Prevalence, Incidence Density, and Genotype Distri... - BV FAPESP
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Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in Sao Paulo, Brazil

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Autor(es):
Giret, Maria Teresa M. [1] ; Miraglia, Joao Luiz [1] ; Araripe Sucupira, Maria Cecilia [2] ; Nishiya, Anna [3] ; Levi, Jose Eduardo [4] ; Diaz, Ricardo S. [2] ; Sabino, Ester C. [3] ; Kallas, Esper G. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Div Clin Immunol & Allergy, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Div Infect Dis, Sao Paulo - Brazil
[3] Sao Paulo Blood Bank, Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 6, n. 4 APR 5 2011.
Citações Web of Science: 20
Resumo

Background: The results of previous studies elsewhere have indicated that GB virus C (GBV-C) infection is frequent in patients infected with the human immunodeficiency virus type 1 (HIV-1) due to similar transmission routes of both viruses. The aim of this study was to determine the prevalence, incidence density and genotypic characteristics of GBV-C in this population. Methodology/Principal Findings: The study population included 233 patients from a cohort primarily comprised of homosexual men recently infected with HIV-1 in Sao Paulo, Brazil. The presence of GBV-C RNA was determined in plasma samples by reverse transcriptase-nested polymerase chain reaction and quantified by real-time PCR. GBV-C genotypes were determined by direct sequencing. HIV viral load, CD4+ T lymphocyte and CD8+ T lymphocyte count were also tested in all patients. The overall prevalence of GBV-C infection was 0.23 (95% CI: 0.18 to 0.29) in the study group. There was no significant difference between patients with and without GBV-C infection and Glycoprotein E2 antibody presence regarding age, sex, HIV-1 viral load, CD4+ and CD8+ T cell counts and treatment with antiretroviral drugs. An inverse correlation was observed between GBV-C and HIV-1 loads at enrollment and after one year. Also, a positive but not significant correlation was observed between GBV-C load and CD4+ T lymphocyte. Phylogenetic analysis of the GBV-C isolates revealed the presence of genotype 1 and genotype 2, these sub classified into subtype 2a and 2b. Conclusion/Significance: GBV-C infection is common in recently HIV -1 infected patients in Sao Paulo, Brazil and the predominant genotype is 2b. This study provides the first report of the GBV-C prevalence at the time of diagnosis of HIV-1 and the incidence density of GBV-C infection in one year. (AU)

Processo FAPESP: 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP
Beneficiário:Ricardo Sobhie Diaz
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 05/01072-9 - A influência da infecção pelo GB virus com virus da hepatite G (HGV) na evolução da doença pelo HIV em mulheres
Beneficiário:José Eduardo Levi
Modalidade de apoio: Auxílio à Pesquisa - Regular