| Full text | |
| Author(s): |
Giret, Maria Teresa M.
[1]
;
Miraglia, Joao Luiz
[1]
;
Araripe Sucupira, Maria Cecilia
[2]
;
Nishiya, Anna
[3]
;
Levi, Jose Eduardo
[4]
;
Diaz, Ricardo S.
[2]
;
Sabino, Ester C.
[3]
;
Kallas, Esper G.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Div Clin Immunol & Allergy, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Div Infect Dis, Sao Paulo - Brazil
[3] Sao Paulo Blood Bank, Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PLoS One; v. 6, n. 4 APR 5 2011. |
| Web of Science Citations: | 20 |
| Abstract | |
Background: The results of previous studies elsewhere have indicated that GB virus C (GBV-C) infection is frequent in patients infected with the human immunodeficiency virus type 1 (HIV-1) due to similar transmission routes of both viruses. The aim of this study was to determine the prevalence, incidence density and genotypic characteristics of GBV-C in this population. Methodology/Principal Findings: The study population included 233 patients from a cohort primarily comprised of homosexual men recently infected with HIV-1 in Sao Paulo, Brazil. The presence of GBV-C RNA was determined in plasma samples by reverse transcriptase-nested polymerase chain reaction and quantified by real-time PCR. GBV-C genotypes were determined by direct sequencing. HIV viral load, CD4+ T lymphocyte and CD8+ T lymphocyte count were also tested in all patients. The overall prevalence of GBV-C infection was 0.23 (95% CI: 0.18 to 0.29) in the study group. There was no significant difference between patients with and without GBV-C infection and Glycoprotein E2 antibody presence regarding age, sex, HIV-1 viral load, CD4+ and CD8+ T cell counts and treatment with antiretroviral drugs. An inverse correlation was observed between GBV-C and HIV-1 loads at enrollment and after one year. Also, a positive but not significant correlation was observed between GBV-C load and CD4+ T lymphocyte. Phylogenetic analysis of the GBV-C isolates revealed the presence of genotype 1 and genotype 2, these sub classified into subtype 2a and 2b. Conclusion/Significance: GBV-C infection is common in recently HIV -1 infected patients in Sao Paulo, Brazil and the predominant genotype is 2b. This study provides the first report of the GBV-C prevalence at the time of diagnosis of HIV-1 and the incidence density of GBV-C infection in one year. (AU) | |
| FAPESP's process: | 04/15856-9 - Prospective analysis of the virological and immunological characteristics in individuals with recent HIV-1 infection in the cities of São Paulo and Santos |
| Grantee: | Ricardo Sobhie Diaz |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 05/01072-9 - GB virus C/HGV and prognosis on HIV infected women |
| Grantee: | José Eduardo Levi |
| Support Opportunities: | Regular Research Grants |