Are endogenous sex hormones related to DNA damage in paradoxically sleep-deprived female rats?

Autor(es)

Andersen, Monica L. ^[1] ; Ribeiro, Daniel A. ^[2] ; Alvarenga, Tathiana A. ^[1] ; Silva, Andressa ^[1] ; Araujo, Paula ^[1] ; Zager, Adriano ^[1] ; Tenorio, Neuli M. ^[1] ; Tufik, Sergio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psychobiol, BR-04024002 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, UNIFESP, BR-04024002 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Hormones and Behavior; v. 57, n. 2, p. 216-221, FEB 2010.
• Citações Web of Science: 9

Resumo

The aim of this investigation was to evaluate overall DNA damage induced by experimental paradoxical sleep deprivation (PSD) in estrous-cycling and ovariectomized female rats to examine possible hormonal involvement during DNA damage. Intact rats in different phases of the estrous cycle (proestrus, estrus, and diestrus) or ovariectomized female Wistar rats were subjected to PSD by the single platform technique for 96 h or were maintained for the equivalent period as controls in home-cages. After this period, peripheral blood and tissues (brain, liver, and heart) were collected to evaluate genetic damage using the single cell gel (comet) assay. The results showed that PSD caused extensive genotoxic effects in brain cells, as evident by increased DNA migration rates in rats exposed to PSD for 96 h when compared to negative control. This was observed for all phases of the estrous cycle indistinctly. In ovariectomized rats, PSD also led to DNA damage in brain cells. No significant statistically differences were detected in peripheral blood, the liver or heart for all groups analyzed. In conclusion, our data are consistent with the notion that genetic damage in the form of DNA breakage in brain cells induced by sleep deprivation overrides the effects related to endogenous female sex hormones. (c) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 09/01030-5 - Efeito da privação e restrição de sono na função reprodutiva de ratos machos
• Beneficiário: Tathiana Aparecida Fernandes de Alvarenga
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/58275-1 - Efeitos da privação de sono na resposta ao lipopolissacarídeo bacteriano: aspectos comportamentais, neuroquímicos e imunológicos em camundongos
• Beneficiário: Adriano Zager
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
• Beneficiário: Daniel Araki Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 98/14303-3 - Center for Sleep Studies
• Beneficiário: Sergio Tufik
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs