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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Are endogenous sex hormones related to DNA damage in paradoxically sleep-deprived female rats?

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Andersen, Monica L. [1] ; Ribeiro, Daniel A. [2] ; Alvarenga, Tathiana A. [1] ; Silva, Andressa [1] ; Araujo, Paula [1] ; Zager, Adriano [1] ; Tenorio, Neuli M. [1] ; Tufik, Sergio [1]
Total Authors: 8
[1] Univ Fed Sao Paulo, Dept Psychobiol, BR-04024002 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, UNIFESP, BR-04024002 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Hormones and Behavior; v. 57, n. 2, p. 216-221, FEB 2010.
Web of Science Citations: 10

The aim of this investigation was to evaluate overall DNA damage induced by experimental paradoxical sleep deprivation (PSD) in estrous-cycling and ovariectomized female rats to examine possible hormonal involvement during DNA damage. Intact rats in different phases of the estrous cycle (proestrus, estrus, and diestrus) or ovariectomized female Wistar rats were subjected to PSD by the single platform technique for 96 h or were maintained for the equivalent period as controls in home-cages. After this period, peripheral blood and tissues (brain, liver, and heart) were collected to evaluate genetic damage using the single cell gel (comet) assay. The results showed that PSD caused extensive genotoxic effects in brain cells, as evident by increased DNA migration rates in rats exposed to PSD for 96 h when compared to negative control. This was observed for all phases of the estrous cycle indistinctly. In ovariectomized rats, PSD also led to DNA damage in brain cells. No significant statistically differences were detected in peripheral blood, the liver or heart for all groups analyzed. In conclusion, our data are consistent with the notion that genetic damage in the form of DNA breakage in brain cells induced by sleep deprivation overrides the effects related to endogenous female sex hormones. (c) 2009 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 98/14303-3 - Center for Sleep Studies
Grantee:Sergio Tufik
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 06/58275-1 - Effects of paradoxical sleep deprivation on response to lipopolyssacharide: immunological, behavioral and neurochemical aspects in mice
Grantee:Adriano Zager
Support type: Scholarships in Brazil - Master
FAPESP's process: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis
Grantee:Daniel Araki Ribeiro
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 09/01030-5 - Effect of sleep deprivation and sleep restriction on the reproductive function of male rats
Grantee:Tathiana Aparecida Fernandes de Alvarenga
Support type: Scholarships in Brazil - Doctorate