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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Structural changes in the epithelium of the small intestine and immune cell infiltration of enteric ganglia following acute mucosal damage and local inflammation

Texto completo
Pontell, Louise [1] ; Castelucci, Patricia [2, 1] ; Bagyanszki, Maria [1, 3] ; Jovic, Tanja [1] ; Thacker, Michelle [1] ; Nurgali, Kulmira [4] ; Bron, Romke [1] ; Furness, John B. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Melbourne, Dept Anat & Cell Biol, Melbourne, Vic 3010 - Australia
[2] Univ Sao Paulo, Dept Anat, Sao Paulo - Brazil
[3] Univ Szeged, Dept Physiol Anat & Neurosci, H-6726 Szeged - Hungary
[4] Univ Melbourne, Dept Physiol, Melbourne, Vic 3010 - Australia
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Virchows Archiv; v. 455, n. 1, p. 55-65, JUL 2009.
Citações Web of Science: 29

An acute enteritis is commonly followed by intestinal neuromuscular dysfunction, including prolonged hyperexcitability of enteric neurons. Such motility disorders are associated with maintained increases in immune cells adjacent to enteric ganglia and in the mucosa. However, whether the commonly used animal model, trinitrobenzene sulphonate (TNBS)-induced enteritis, causes histological and immune cell changes similar to human enteric neuropathies is not clear. We have made a detailed study of the mucosal damage and repair and immune cell invasion following intralumenal administration of TNBS. Intestines from untreated, sham-operated and TNBS-treated animals were examined at 3 h to 56 days. At 3 h, the mucosal surface was completely ablated, by 6 h an epithelial covering was substantially restored and by 1 day there was full re-epithelialisation. The lumenal epithelium developed from a squamous cell covering to a fully differentiated columnar epithelium with mature villi at about 7 days. Prominent phagocytic activity of enterocytes occurred at 1-7 days. A surge of eosinophils and T lymphocytes associated with the enteric nerve ganglia occurred at 3 h to 3 days. However, elevated immune cell numbers occurred in the lamina propria of the mucosa until 56 days, when eosinophils were still three times normal. We conclude that the disruption of the mucosal surface that causes TNBS-induced ileitis is brief, a little more than 6 h, and causes a transient immune cell surge adjacent to enteric ganglia. This is much briefer than the enteric neuropathy that ensues. Ongoing mucosal inflammatory reaction may contribute to the persistence of enteric neuropathy. (AU)

Processo FAPESP: 08/05718-9 - Reações de tipos de células específicas no gânglio entérico à isquemia intestinal
Beneficiário:Patricia Castelucci
Linha de fomento: Bolsas no Exterior - Pesquisa