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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Structural changes in the epithelium of the small intestine and immune cell infiltration of enteric ganglia following acute mucosal damage and local inflammation

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Author(s):
Pontell, Louise [1] ; Castelucci, Patricia [2, 1] ; Bagyanszki, Maria [1, 3] ; Jovic, Tanja [1] ; Thacker, Michelle [1] ; Nurgali, Kulmira [4] ; Bron, Romke [1] ; Furness, John B. [1]
Total Authors: 8
Affiliation:
[1] Univ Melbourne, Dept Anat & Cell Biol, Melbourne, Vic 3010 - Australia
[2] Univ Sao Paulo, Dept Anat, Sao Paulo - Brazil
[3] Univ Szeged, Dept Physiol Anat & Neurosci, H-6726 Szeged - Hungary
[4] Univ Melbourne, Dept Physiol, Melbourne, Vic 3010 - Australia
Total Affiliations: 4
Document type: Journal article
Source: Virchows Archiv; v. 455, n. 1, p. 55-65, JUL 2009.
Web of Science Citations: 29
Abstract

An acute enteritis is commonly followed by intestinal neuromuscular dysfunction, including prolonged hyperexcitability of enteric neurons. Such motility disorders are associated with maintained increases in immune cells adjacent to enteric ganglia and in the mucosa. However, whether the commonly used animal model, trinitrobenzene sulphonate (TNBS)-induced enteritis, causes histological and immune cell changes similar to human enteric neuropathies is not clear. We have made a detailed study of the mucosal damage and repair and immune cell invasion following intralumenal administration of TNBS. Intestines from untreated, sham-operated and TNBS-treated animals were examined at 3 h to 56 days. At 3 h, the mucosal surface was completely ablated, by 6 h an epithelial covering was substantially restored and by 1 day there was full re-epithelialisation. The lumenal epithelium developed from a squamous cell covering to a fully differentiated columnar epithelium with mature villi at about 7 days. Prominent phagocytic activity of enterocytes occurred at 1-7 days. A surge of eosinophils and T lymphocytes associated with the enteric nerve ganglia occurred at 3 h to 3 days. However, elevated immune cell numbers occurred in the lamina propria of the mucosa until 56 days, when eosinophils were still three times normal. We conclude that the disruption of the mucosal surface that causes TNBS-induced ileitis is brief, a little more than 6 h, and causes a transient immune cell surge adjacent to enteric ganglia. This is much briefer than the enteric neuropathy that ensues. Ongoing mucosal inflammatory reaction may contribute to the persistence of enteric neuropathy. (AU)

FAPESP's process: 08/05718-9 - Enteric ganglia specific cell type reactions to intestinal ischemia
Grantee:Patricia Castelucci
Support type: Scholarships abroad - Research