Prospective study of potential sources of Streptococcus mutans transmission in nursery school children

Autor(es)

Alves, Alessandra C. ^[1] ; Nogueira, Ruchele D. ^[1] ; Stipp, Rafael N. ^[1] ; Pampolini, Flavia ^[1] ; Moraes, Antonio B. A. ^[2] ; Goncalves, Reginaldo B. ^[1] ; Hoefling, Jose F. ^[1] ; Li, Yihong ^[3] ; Mattos-Graner, Renata O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Ctr Treatment Special Patients CEPAE, Sao Paulo - Brazil

^[3] New York Coll Dent, Dept Basic Sci & Craniofacial Biol, New York, NY - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Microbiology; v. 58, n. 4, p. 476-481, APR 2009.
• Citações Web of Science: 27

Resumo

Transmission of Streptococcus mutans, a major dental caries pathogen, occurs mainly during the first 2.5 years of age. Children appear to acquire S. mutans mostly from their mothers, but few studies have investigated non-familial sources of S. mutans transmission. This study prospectively analysed initial S. mutans oral colonization in 119 children from nursery schools during a 1.5-year period and tracked the transmission from child to child, day-care caregiver to child and mother to child. Children were examined at baseline, when they were 5-13 months of age, and at 6-month intervals for determination of oral levels of S. mutans and development of caries lesions. Levels of S. mutans were also determined in caregivers and mothers. A total of 1392 S. mutans isolates (obtained from children, caregivers and mothers) were genotyped by arbitrarily primed PCR and chromosomal RFLP. Overall, 40.3% of children were detectably colonized during the study, and levels of S. mutans were significantly associated with the development of caries lesions. Identical S. mutans genotypes were found in four nursery cohorts. No familial relationship existed in three of these cohorts, indicating horizontal transmission. Despite high oral levels of S. mutans identified in most of the caregivers, none of their genotypes matched those identified in the respective children. Only 50% of children with high levels of S. mutans carried genotypes identified in their mothers. The results support previous evidence indicating that non-familial sources of S. mutans transmission exist, and indicate that this bacterium may be transmitted horizontally between children during the initial phases of S. mutans colonization in nursery environments. (AU)

• Processo FAPESP: 02/07156-1 - Análise da resposta imunológica de crianças durante a colonização por Streptococcus mutans e dos fatores genético-moleculares envolvidos na formação do biofilme dental por estes microrganismos
• Beneficiário: Renata de Oliveira Mattos Graner
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 03/01836-3 - Análise da resposta imunológica de crianças durante a colonização por Streptococcus mutans e dos fatores genético-moleculares envolvidos na formação do biofilme dental por estes microrganismos
• Beneficiário: Renata de Oliveira Mattos Graner
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores