In Vivo Effects of Bothrops jararaca Venom on Metabolic Profile and on Muscle Protein Metabolism in Rats

Autor(es)

Goncalves, Dawit A. P. ^[1] ; Silva, Erico V. C. M. ^[2] ; Graca, Flavia A. ^[1] ; Lira, Eduardo C. ^[1] ; Zanon, Neusa M. ^[1] ; Mendes, Gloria E. ; Burdmann, Emmanuel A. ^{[3, 4]} ; Migliorini, Renato H. ^[1] ; Kettelhut, Isis C. ^[1] ; Navegantes, Luiz C. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, BR-14049900 Ribeirao Preto - Brazil

^[2] UNESP, Sch Med Botucatu, Dept Ophthalmol, BR-18618000 Botucatu, SP - Brazil

^[3] Sch Med Sao Jose do Rio Preto, Dept Mol Biol, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[4] Sch Med Sao Jose do Rio Preto, Div Nephrol, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Tropical Medicine and Hygiene; v. 79, n. 5, p. 771-778, NOV 2008.
• Citações Web of Science: 6

Resumo

This study investigated the in vivo effects of the Bothrops Jararaca venom (BjV) on general metabolic profile and, specifically. oil muscle protein metabolism in rats. The crude venom (0.4 mg/kg body weight, IV) was infused in awake rats, and plasma activity of enzymes and metabolites levels were determined after 1, 2, 3, and 4 hours. BjV increased urea, lactate, and activities of creatine kinase. lactate dehydrogenase. and aspartate aminotransferase after 4 hours. The content of liver glycogen was reduced by BjV. Protein metabolism was evaluated by means of microdialysis technique and in isolated muscles. BjV induced increase in the muscle interstitial-arterial tyrosine concentration difference. indicating a high protein catabolism. The myotoxicity induced by this venom is associated with reduction of protein synthesis and increase in rates of overall proteolysis, which was accompanied by activation of lysosomal and ubiquitin-proteasome systems without changes in protein levels of cathepsins and ubiquitin-protein conjugates. (AU)

• Processo FAPESP: 04/02674-0 - Controle da perda de proteínas musculares em situações catabólicas: um estudo de microdiálise
• Beneficiário: Luiz Carlos Carvalho Navegantes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores