gamma-Radiation induces apoptosis via sarcoplasmatic reticulum in guinea pig ileum smooth muscle cells

Autor(es)

Claro, Sandra ^[1] ; Miyamoto Oshiro, Maria Etsuko ^[1] ; Freymuller, Edna ; Katchburian, Eduardo ^{[2, 3]} ; Kallas, Esper George ^[4] ; Cerri, Paulo Sergio ^[5] ; Ferreira, Alice Teixeira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biophys, EPM, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Ctr Electron Microscopy, EPM, BR-04023062 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Morphol, EPM, BR-04023062 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Gene Therapy, EPM, BR-04023062 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Dept Morphol, Sch Dent, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Pharmacology; v. 590, n. 1-3, p. 20-28, AUG 20 2008.
• Citações Web of Science: 6

Resumo

We investigated the effects of gamma-radiation on cells isolated from the longitudinal smooth muscle layer of the guinea pig ileum, a relatively radioresistant tissue. Single doses (up to 50 Gy) reduced the amount of sarcoplasmatic reticulum and condensed the myofibrils, as shown by electron microscopy 3 days post-irradiation. After that, contractility of smooth muscle strips was reduced. Ca(2+) handling was altered after irradiation, as shown in fura-2 loaded cells, with elevated basal intracellular Ca(2+), reduced amount of intrareticular Ca(2+), and reduced capacitive Ca(2+) entry. Radiation also induced apoptosis, judged from flow cytometry of cells loaded with proprium iodide. Electron microscopy showed that radiation caused condensation of chromatin in dense masses around the nuclear envelope, the presence of apoptotic bodies, fragmentation of the nucleus, detachment of cells from their neighbors, and reductions in cell volume. Radiation also caused activation of caspase 12. Apoptosis was reduced by the administration of the caspase inhibitor Z-Val-Ala-Asp-fluoromethyl-ketone methyl ester (Z-VAD-FIVIK) during the 3 day period after irradiation, and by the chelator of intracellular Ca(2+), 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), from 1 h before until 2 h after irradiation. BAPTA also reduced the effects of radiation on contractility, basal intracellular Ca(2+), amount of intrareticular Ca(2+), capacitative Ca(2+) entry, and apoptosis. In conclusion, the effects of gamma radiation on contractility, Ca(2+) handling, and apoptosis appear due to a toxic action of intracellular Ca(2+). Ca(2+)-induced damage to the sarcoplasmatic reticulum seems a key event in impaired Ca(2+) handling and apoptosis induced by gamma-radiation. (c) 2008 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 99/10261-7 - Estudo da sinalização, proliferação e transformação celular: redistribuição e compartimentalização de íons receptores e proteoglicanos
• Beneficiário: Alice Teixeira Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários