Effects of Etch-and-Rinse and Self-etch Adhesives on Dentin MMP-2 and MMP-9

Autor(es)

Mazzoni, A. ^[1] ; Scaffa, P. ^[2] ; Carrilho, M. ^[3] ; Tjaederhane, L. ^[4] ; Di Lenarda, R. ^[1] ; Polimeni, A. ^[5] ; Tezvergil-Mutluay, A. ^[6] ; Tay, F. R. ^{[7, 8]} ; Pashley, D. H. ^[6] ; Breschi, L. ^{[1, 9]}

Afiliação do(s) autor(es)

^[1] Univ Trieste, Unit Dent Sci & Biomat, Dept Biomed, Trieste - Italy

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba - Brazil

^[3] Univ Western Ontario, Sch Dent, Schulich Sch Med & Dent, London, ON - Canada

^[4] Univ Oulu, Inst Dent, Oulu Univ Hosp, Oulu - Finland

^[5] Univ Roma La Sapienza, Dept Oral & Maxillofacial Sci, Rome - Italy

^[6] Georgia Hlth Sci Univ, Dept Oral Biol & Maxillofacial Pathol, Sch Dent, Augusta, GA - USA

^[7] Univ Turku, Adhes Dent Res Grp, Dept Prosthet Dent, SF-20500 Turku - Finland

^[8] Turku Univ Hosp, TYKS, FIN-20520 Turku - Finland

^[9] IOR, IGM CNR, Unit Bologna, Bologna - Italy

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DENTAL RESEARCH; v. 92, n. 1, p. 82-86, JAN 2013.
• Citações Web of Science: 73

Resumo

Auto-degradation of collagen matrices occurs within hybrid layers created by contemporary dentin bonding systems, by the slow action of host-derived matrix metalloproteinases (MMPs). This study tested the null hypothesis that there are no differences in the activities of MMP-2 and -9 after treatment with different etch-and-rinse or self-etch adhesives. Tested adhesives were: Adper Scotchbond 1XT (3M ESPE), PQ1 (Ultradent), Peak LC (Ultradent), Optibond Solo Plus (Kerr), Prime\&Bond NT (Dentsply) (all 2-step etch-and-rinse adhesives), and Adper Easy Bond (3M ESPE), Tri-S (Kuraray), and Xeno-V (Dentsply) (1-step self-etch adhesives). MMP-2 and -9 activities were quantified in adhesive-treated dentin powder by means of an activity assay and gelatin zymography. MMP-2 and MMP-9 activities were found after treatment with all of the simplified etch-and-rinse and self-etch adhesives; however, the activation was adhesive-dependent. It is concluded that all two-step etch-and-rinse and the one-step self-etch adhesives tested can activate endogenous MMP-2 and MMP-9 in human dentin. These results support the role of endogenous MMPs in the degradation of hybrid layers created by these adhesives. (AU)

• Processo FAPESP: 09/14005-9 - Efeito de inibidores de protease na solubilização da dentina desmineralizada, na preservação das propriedades mecânicas da matriz de dentina e na durabilidade das restaurações adesivas.
• Beneficiário: Polliana Mendes Candia Scaffa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/54618-4 - Avaliação da atividade de colagenases na degradação da dentina humana tratada por procedimentos restauradores adesivos
• Beneficiário: Marcela Rocha de Oliveira Carrilho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores