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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Etch-and-Rinse and Self-etch Adhesives on Dentin MMP-2 and MMP-9

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Author(s):
Mazzoni, A. [1] ; Scaffa, P. [2] ; Carrilho, M. [3] ; Tjaederhane, L. [4] ; Di Lenarda, R. [1] ; Polimeni, A. [5] ; Tezvergil-Mutluay, A. [6] ; Tay, F. R. [7, 8] ; Pashley, D. H. [6] ; Breschi, L. [1, 9]
Total Authors: 10
Affiliation:
[1] Univ Trieste, Unit Dent Sci & Biomat, Dept Biomed, Trieste - Italy
[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba - Brazil
[3] Univ Western Ontario, Sch Dent, Schulich Sch Med & Dent, London, ON - Canada
[4] Univ Oulu, Inst Dent, Oulu Univ Hosp, Oulu - Finland
[5] Univ Roma La Sapienza, Dept Oral & Maxillofacial Sci, Rome - Italy
[6] Georgia Hlth Sci Univ, Dept Oral Biol & Maxillofacial Pathol, Sch Dent, Augusta, GA - USA
[7] Univ Turku, Adhes Dent Res Grp, Dept Prosthet Dent, SF-20500 Turku - Finland
[8] Turku Univ Hosp, TYKS, FIN-20520 Turku - Finland
[9] IOR, IGM CNR, Unit Bologna, Bologna - Italy
Total Affiliations: 9
Document type: Journal article
Source: JOURNAL OF DENTAL RESEARCH; v. 92, n. 1, p. 82-86, JAN 2013.
Web of Science Citations: 73
Abstract

Auto-degradation of collagen matrices occurs within hybrid layers created by contemporary dentin bonding systems, by the slow action of host-derived matrix metalloproteinases (MMPs). This study tested the null hypothesis that there are no differences in the activities of MMP-2 and -9 after treatment with different etch-and-rinse or self-etch adhesives. Tested adhesives were: Adper Scotchbond 1XT (3M ESPE), PQ1 (Ultradent), Peak LC (Ultradent), Optibond Solo Plus (Kerr), Prime\&Bond NT (Dentsply) (all 2-step etch-and-rinse adhesives), and Adper Easy Bond (3M ESPE), Tri-S (Kuraray), and Xeno-V (Dentsply) (1-step self-etch adhesives). MMP-2 and -9 activities were quantified in adhesive-treated dentin powder by means of an activity assay and gelatin zymography. MMP-2 and MMP-9 activities were found after treatment with all of the simplified etch-and-rinse and self-etch adhesives; however, the activation was adhesive-dependent. It is concluded that all two-step etch-and-rinse and the one-step self-etch adhesives tested can activate endogenous MMP-2 and MMP-9 in human dentin. These results support the role of endogenous MMPs in the degradation of hybrid layers created by these adhesives. (AU)

FAPESP's process: 09/14005-9 - Effect of protease inhibitors on the solubilization of demineralized dentin, preservation of mechanical properties of dentin matrix and durability of adhesive restorations.
Grantee:Polliana Mendes Candia Scaffa
Support Opportunities: Scholarships in Brazil - Doctorate