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Effect of bioactive glass-ceramic associated to natural primers on dentin-adhesive interface


The search for treatments that improve the longevity of the dentin/adhesive interface is constant. The success of the esthetic treatment is related to the lifetime of the restorations that can be influenced by several factors such as the materials, the clinical time of use and the dentin matrix degradation mediated by matrix metalloproteinases (MMPs). Also, the use of a remineralizing material would be indicated to decrease the injuries on dentin tissue. Thus, searching for better results in durability of adhesive restorations, the aim of this study is to evaluate the effect of the use of Biosilicate solution associated with natural therapeutic primers on bond strength (RU) of composite restorations to the dentin. For this, 320 healthy human molars will be selected to the study, that will have their occlusal surface ground flat and in the central area of the tooth surface, Class I cavities will be prepared (5 mm length, 4 mm width and 4 mm depth) using carbide burs. Half of the samples will be submitted to Des-Re protocols for the development of artificial caries. Afterward, all teeth will be separated into eight groups (n = 20) according to the treatment received before adhesive system (Adper Single Bond Universal - 3M ESPE) be used: Control Group: Adhesive System; CHX Group - Chlorhexidine (CHX) 0.12%; Bio Group - Biosilicate solution at 10%; P16 Group - Propolis extract with low levels of polyphenols; P45 Group - Propolis extract with high levels of polyphenols; CHXBio Group - CHX+Biosilicate; P16Bio - Propolis extract with low levels of polyphenols+Biosilicate; P45Bio - Propolis extract with high levels of polyphenols + Biosilicate. After restorative procedures, the samples will be sectioned into sticks in accordance with the "non-trimming" technique and will be separated and storage in distilled and deionized water at 37ºC for 24h, 6 months and 1 year. After this periods of storage, the sticks will be submitted to microtensile test (0.5mm/min). The fracture patterns will be analyzed by SEM (JSM 5410, Sony). The degradation of the dentin-adhesive interface after the treatments will be analyzed by Transmission electronic microscopy (TEM, n=2), in situ zymography (n=2) and by Energy-dispersive X-ray analysis (EDX, n=2). The biostability of dentin matrix treated will be evaluated by enzymatic biodegradation assay. After data collection, they will be submitted to statistical analysis according to the most appropriate statistical test. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VIVANCO, ROCIO GENG; TONANI-TORRIERI, RAFAELLA; SOUZA, ANA BEATRIZ SILVA; MARQUELE-OLIVEIRA, FRANCIANE; PIRES-DE-SOUZA, FERNANDA DE CARVALHO PANZERI. Effect of natural primer associated to bioactive glass-ceramic on adhesive/ dentin interface. Journal of Dentistry, v. 106, . (17/14417-1, 18/07376-0)

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