Impact of Protease Inhibitors on Dentin Matrix Degradation by Collagenase

Autor(es)

Kato, M. T. ^[1] ; Leite, A. L. ^[1] ; Hannas, A. R. ^[1] ; Calabria, M. P. ^[2] ; Magalhaes, A. C. ^[1] ; Pereira, J. C. ^[2] ; Buzalaf, M. A. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, BR-17012901 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DENTAL RESEARCH; v. 91, n. 12, p. 1119-1123, DEC 2012.
• Citações Web of Science: 44

Resumo

This proof-of-concept study assessed whether the reduction of the degradation of the demineralized organic matrix (DOM) by pre-treatment with protease inhibitors (PI) is effective against dentin matrix loss. Bovine dentin slices were demineralized with 0.87 M citric acid, pH 2.3, for 36 hrs. In sequence, specimens were treated or not (UT, untreated) for 1 min with gels containing epigallocatechin 3-gallate (EGCG, 400 A mu M), chlorhexidine (CHX, 0.012%), FeSO4 (1 mM), NaF (1.23%), or no active compound (P, placebo). Specimens were then stored in artificial saliva (5 days, 37 degrees C) with the addition of collagenase (Clostridium histolyticum, 100 U/mL). We analyzed collagen degradation by assaying hydroxyproline (HYP) in the incubation solutions (n = 5) and evaluated the dentin matrix loss by profilometry (n = 12). Data were analyzed by ANOVA and Tukey's test (p < 0.05). Treatment with gels containing EGCG, CHX, or FeSO4 led to significantly lower HYP concentrations in solution and dentin matrix loss when compared with the other treatments. These results strongly suggest that the preventive effects of the PI tested against dentin erosion are due to their ability to reduce the degradation of the DOM. (AU)

• Processo FAPESP: 07/04209-0 - Avaliação do efeito do chá verde, inibidor natural das metaloproteinases de matriz, sobre a erosão da dentina associada ou não à abrasão: um estudo in situ
• Beneficiário: Melissa Thiemi Kato
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/08389-3 - Avaliação do efeito de inibidores de metaloproteinases de matriz sobre a erosão dentária: estudos in situ / ex vivo
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular