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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of Protease Inhibitors on Dentin Matrix Degradation by Collagenase

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Author(s):
Kato, M. T. [1] ; Leite, A. L. [1] ; Hannas, A. R. [1] ; Calabria, M. P. [2] ; Magalhaes, A. C. [1] ; Pereira, J. C. [2] ; Buzalaf, M. A. R. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, BR-17012901 Bauru, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF DENTAL RESEARCH; v. 91, n. 12, p. 1119-1123, DEC 2012.
Web of Science Citations: 44
Abstract

This proof-of-concept study assessed whether the reduction of the degradation of the demineralized organic matrix (DOM) by pre-treatment with protease inhibitors (PI) is effective against dentin matrix loss. Bovine dentin slices were demineralized with 0.87 M citric acid, pH 2.3, for 36 hrs. In sequence, specimens were treated or not (UT, untreated) for 1 min with gels containing epigallocatechin 3-gallate (EGCG, 400 A mu M), chlorhexidine (CHX, 0.012%), FeSO4 (1 mM), NaF (1.23%), or no active compound (P, placebo). Specimens were then stored in artificial saliva (5 days, 37 degrees C) with the addition of collagenase (Clostridium histolyticum, 100 U/mL). We analyzed collagen degradation by assaying hydroxyproline (HYP) in the incubation solutions (n = 5) and evaluated the dentin matrix loss by profilometry (n = 12). Data were analyzed by ANOVA and Tukey's test (p < 0.05). Treatment with gels containing EGCG, CHX, or FeSO4 led to significantly lower HYP concentrations in solution and dentin matrix loss when compared with the other treatments. These results strongly suggest that the preventive effects of the PI tested against dentin erosion are due to their ability to reduce the degradation of the DOM. (AU)

FAPESP's process: 07/08389-3 - Evaluation of the effect of matrix metalloproteinases inhibitors on dental erosion: in situ / ex vivo studies
Grantee:Marília Afonso Rabelo Buzalaf
Support Opportunities: Regular Research Grants