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Inhibitory effect of organic matrix degradation and wear of Cranberry and proanthocyanidin gels on dentin submitted to dental erosion

Grant number: 14/26754-4
Support Opportunities:Regular Research Grants
Duration: June 01, 2015 - May 31, 2017
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Heitor Marques Honório
Grantee:Heitor Marques Honório
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


There are indications that the Cranberry and proanthocyanidin (its active ingredient) have the potential to inhibit matrix metalloproteinases (MMPs) that degrade the dentin collagen, but these agents have not been properly evaluated.The objective of this study is to evaluate the role of Cranberry extract and its main active agent (proanthocyanidin) applied as a topical gel in the inhibition of the degradation of the organic matrix of demineralized dentin and thus minimizing the dentin wear subjected to erosion. Two projects will be performed. For the first project, dentin specimens (4 mm thick) in 5 ml of demineralized citric acid 0.87 M, pH 2.3, for 36 h at 4 ° C are used.Then, the specimens will be divided and submitted to the protocol of the 5 experimental groups of this study: G1 specimens treated with proanthocyanidin gel 10% (test 1), G2 specimens treated with Cranberry gel 10% (test 2) G3 specimens treated with chlorhexidine gel 0.012% (positive control 1), G4 specimens treated with 1.23% NaF gel (positive control 2) and G5 specimens treated with placebo gel (negative control 1). Each group will be demineralized collagen degradation by the action of collagenase from Clostridium histolyticum (100 U / ml), added with 5 ml of artificial saliva containing EDTA- free protease inhibitor for 5 days at 37 ° C. The samples are 1000 X concentrated HCl and hydrolyzed with 6 mol / ml at 98oC for 24 hours. Will again lyophilized and resuspended in water (600 ul) and then the levels of hydroxyproline is performed (Method 1.Assessment of loss of dentin matrix will be made by profilometry (Method 2). In the second project, wear will be evaluated by the following groups: G1 and G6 - dentin specimens treated with 0.05% proanthocyanidin gel during 1 and 5 minutes, respectively, G2 and G7 - dentin specimens treated with 1% proanthocyanidin gel during 1 and 5 minutes, respectively, G3 and G8 - dentin specimens treated with 5% proanthocyanidin gel during 1 and 5 minutes, respectively, G4 and G9 - dentin specimens treated with 10% proanthocyanidin gel during 1 and 5 minutes, respectively, G5 and G10 - dentin specimens treated with placebo gel during 1 and 5 minutes, respectively. The data will be submitted to ANOVA followed by Tukey test using p0.05. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BOTEON, ANA PAULA; PRAKKI, ANURADHA; RABELO BUZALAF, MARILIA AFONSO; RIOS, DANIELA; HONORIO, HEITOR MARQUES. Effect of different concentrations and application times of proanthocyanidin gels on dentin erosion. AMERICAN JOURNAL OF DENTISTRY, v. 30, n. 2, p. 96-100, . (14/26754-4)
BOTEON, ANA PAULA; KATO, MELISSA THIEMI; RABELO BUZALAF, MARILIA AFONSO; PRAKKI, ANURADHA; WANG, LINDA; RIOS, DANIELA; HONORIO, HEITOR MARQUES. Effect of Proanthocyanidin-enriched extracts on the inhibition of wear and degradation of dentin demineralized organic matrix. ARCHIVES OF ORAL BIOLOGY, v. 84, p. 118-124, . (14/26754-4, 14/25833-8)

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