Topoisomerase expression in oral squamous cell carcinoma: relationship with cancer stem cells profiles and lymph node metastasis

Autor(es)

Oliveira-Costa, Joao Paulo ^{[1, 2]} ; Oliveira, Lucinei Roberto ^[1] ; da Silveira, Giorgia Gobbi ^[1] ; Soave, Danilo Figueiredo ^[1] ; Soares, Fernando Augusto ^[2] ; Ribeiro-Silva, Alfredo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol, BR-14049900 Sao Paulo - Brazil

^[2] Antonio Prudente Fdn, AC Camargo Canc Hosp, Dept Pathol Anat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL PATHOLOGY & MEDICINE; v. 41, n. 10, p. 762-768, NOV 2012.
• Citações Web of Science: 6

Resumo

BACKGROUND: The relationship between predictive proteins and tumors presenting cancer stem cells (CSCs) profiles in oral tumors is still poorly understood. This study aims to identify the relationship between topoisomerases I, II alpha, and III alpha and putative CSCs immunophenotype in oral squamous cell carcinoma (OSCC) and determine its influence on prognosis. METHODS: The following data were retrieved from 127 patients: age, gender, primary anatomic site, smoking and alcohol intake, recurrence, metastases, histologic classification, treatment, and survival. An immunohistochemical study for topoisomerases I, II alpha, and III alpha was performed in a tissue microarray containing 127 paraffin blocks of OSCCs. RESULTS: In univariate analysis, topoisomerases expression showed significant differences according to CSCs profiles and p53 immunoexpression, but not with survival. Topoisomerases II alpha and III alpha also showed significant relationship with lymph node metastasis. The multivariate test confirmed these associations. CONCLUSIONS: The results that all topoisomerases correlates with OSCC CSCs may indicate a role for topoisomerases in head and neck carcinogenesis. Notwithstanding, it is plausible that other members of topoisomerases family could represent novel therapeutical targets in oral squamous cell carcinoma. J Oral Pathol Med (2012) 41: 762-768 (AU)

• Processo FAPESP: 10/08400-0 - Identificação de padrões moleculares relacionados ao desenvolvimento do carcinoma epidermóide oral utilizando a tecnologia de micro-arranjos de cDNA
• Beneficiário: João Paulo Oliveira da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado