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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Increased Metallothionein I/II Expression in Patients with Temporal Lobe Epilepsy

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Autor(es):
Peixoto-Santos, Jose Eduardo [1] ; Galvis-Alonso, Orfa Yineth [2] ; Velasco, Tonicarlo Rodrigues [1] ; Kandratavicius, Ludmyla [1] ; Assirati, Joao Alberto [3] ; Carlotti, Carlos Gilberto [3] ; Scandiuzzi, Renata Caldo [1] ; Serafini, Luciano Neder [4] ; Leite, Joao Pereira [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Neurosci & Behav, Ribeirao Preto Sch Med, BR-14049 Ribeirao Preto - Brazil
[2] Sao Jose do Rio Preto Med Sch, Dept Mol Biol, Sao Jose Do Rio Preto - Brazil
[3] Univ Sao Paulo, Dept Neurosurg, Ribeirao Preto Sch Med, BR-14049 Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Dept Pathol, Ribeirao Preto Sch Med, BR-14049 Ribeirao Preto - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 7, n. 9 SEP 18 2012.
Citações Web of Science: 17
Resumo

In the central nervous system, zinc is released along with glutamate during neurotransmission and, in excess, can promote neuronal death. Experimental studies have shown that metallothioneins I/II (MT-I/II), which chelate free zinc, can affect seizures and reduce neuronal death after status epilepticus. Our aim was to evaluate the expression of MT-I/II in the hippocampus of patients with temporal lobe epilepsy (TLE). Hippocampi from patients with pharmacoresistant mesial temporal lobe epilepsy (MTLE) and patients with TLE associated with tumor or dysplasia (TLE-TD) were evaluated for expression of MT-I/II, for the vesicular zinc levels, and for neuronal, astroglial, and microglial populations. Compared to control cases, MTLE group displayed widespread increase in MT-I/II expression, astrogliosis, microgliosis and reduced neuronal population. In TLE-TD, the same changes were observed, except that were mainly confined to fascia dentata. Increased vesicular zinc was observed only in the inner molecular layer of MTLE patients, when compared to control cases. Correlation and linear regression analyses indicated an association between increased MT-I/II and increased astrogliosis in TLE. MT-I/II levels did not correlate with any clinical variables, but MTLE patients with secondary generalized seizures (SGS) had less MT-I/II than MTLE patients without SGS. In conclusion, MT-I/II expression was increased in hippocampi from TLE patients and our data suggest that it is associated with astrogliosis and may be associated with different seizure spread patterns. (AU)

Processo FAPESP: 08/52657-5 - Estudo do padrão da gliose e da expressão das metalotioneínas I/II e da aquaporina-4 no hipocampo de pacientes com epilepsia do lobo temporal
Beneficiário:José Eduardo Peixoto Santos
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/53447-7 - Mecanismos sinápticos e não-sinápticos das epilepsias refratárias e suas implicações na busca de novas estratégias terapêuticas: uma abordagem translacional
Beneficiário:João Pereira Leite
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos
Beneficiário:João Pereira Leite
Linha de fomento: Auxílio à Pesquisa - Programa CINAPCE - Temático