In vitro and in vivo investigation of the biological and mechanical behaviour of resin-modified glass-ionomer cement containing chlorhexidine

Autor(es)

Freire de Castilho, Aline Rogeria ^[1] ; Duque, Cristiane ^[2] ; Negrini, Thais de Cassia ^[3] ; Sacono, Nancy Tomoko ^[4] ; de Paula, Andreia Bolzan ^{[1, 5]} ; de Souza Costa, Carlos Alberto ^[4] ; Palomari Spolidorio, Denise Madalena ^[4] ; Puppin-Rontani, Regina Maria ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Pediat Dent, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo State UNESP, Aracatuba Sch Dent, Dept Pediat Dent, Sao Paulo - Brazil

^[3] Univ Sao Paulo State UNESP, Araraquara Sch Pharm, Dept Clin Anal, Sao Paulo - Brazil

^[4] Univ Sao Paulo State UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Sao Paulo - Brazil

^[5] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Dent Mat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Dentistry; v. 41, n. 2, p. 155-163, FEB 2013.
• Citações Web of Science: 19

Resumo

Objectives: To evaluate: (1) the in vitro antibacterial, cytotoxic and mechanical properties of a resin-modified glass ionomer cement (RMGIC) containing different concentrations of chlorhexidine (CHX) and (2) the in vivo microbiologic action of the best concentration of CHX associated with the RMGIC applied on remaining dentine after indirect pulp treatment (IPT). Methods: For the in vitro studies, RMGIC was associated with 0.2, 0.5, 1.25 and 2.5% CHX. Microbiologic evaluation consisted of an agar diffusion test on cariogenic bacteria for 24 h. Odontoblast-like cell metabolism and morphology analyses measured the cytotoxic effects of the RMGIC groups after 24 h. The same groups were submitted to compressive and diametral tensile strength. The in vivo treatment consisted of IPT using an RMGIC associated with the best CHX concentration. Clinical and microbiologic evaluations were performed before and after 3 months. Results: The use of 1.25% CHX significantly improved the antibacterial effects of the evaluated RMGIC, without causing any detrimental effects to the odontoblast-like cells and on the mechanical properties. This RMGIC and CHX combination completely eliminated mutans streptococci after 3 months of IPT. Conclusion: The RMGIC and 1.25% CHX mixture showed great biological and mechanical behaviour and could be a good treatment against caries progression. Clinical significance: The association of CHX with a liner RMGIC opens a new perspective for arresting residual caries after IPT. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/02606-5 - Avaliação das propriedades biológicas e físico-mecânicas de cimentos de ionômero de vidro associados à clorexidina ou à doxicilina como materiais forradores em procedimentos de remoção parcial de cárie
• Beneficiário: Regina Maria Puppin Rontani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/00359-0 - Avaliação das propriedades biológicas e físico-mecânicas de cimentos de ionômero de vidro associados à clorexidina ou à doxicilina como materiais forradores em procedimentos de remoção parcial de cárie
• Beneficiário: Aline Rogéria Freire de Castilho
• Modalidade de apoio: Bolsas no Brasil - Doutorado