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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro and in vivo investigation of the biological and mechanical behaviour of resin-modified glass-ionomer cement containing chlorhexidine

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Author(s):
Freire de Castilho, Aline Rogeria [1] ; Duque, Cristiane [2] ; Negrini, Thais de Cassia [3] ; Sacono, Nancy Tomoko [4] ; de Paula, Andreia Bolzan [1, 5] ; de Souza Costa, Carlos Alberto [4] ; Palomari Spolidorio, Denise Madalena [4] ; Puppin-Rontani, Regina Maria [1, 5]
Total Authors: 8
Affiliation:
[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Pediat Dent, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Sao Paulo State UNESP, Aracatuba Sch Dent, Dept Pediat Dent, Sao Paulo - Brazil
[3] Univ Sao Paulo State UNESP, Araraquara Sch Pharm, Dept Clin Anal, Sao Paulo - Brazil
[4] Univ Sao Paulo State UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Sao Paulo - Brazil
[5] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Dent Mat, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Dentistry; v. 41, n. 2, p. 155-163, FEB 2013.
Web of Science Citations: 19
Abstract

Objectives: To evaluate: (1) the in vitro antibacterial, cytotoxic and mechanical properties of a resin-modified glass ionomer cement (RMGIC) containing different concentrations of chlorhexidine (CHX) and (2) the in vivo microbiologic action of the best concentration of CHX associated with the RMGIC applied on remaining dentine after indirect pulp treatment (IPT). Methods: For the in vitro studies, RMGIC was associated with 0.2, 0.5, 1.25 and 2.5% CHX. Microbiologic evaluation consisted of an agar diffusion test on cariogenic bacteria for 24 h. Odontoblast-like cell metabolism and morphology analyses measured the cytotoxic effects of the RMGIC groups after 24 h. The same groups were submitted to compressive and diametral tensile strength. The in vivo treatment consisted of IPT using an RMGIC associated with the best CHX concentration. Clinical and microbiologic evaluations were performed before and after 3 months. Results: The use of 1.25% CHX significantly improved the antibacterial effects of the evaluated RMGIC, without causing any detrimental effects to the odontoblast-like cells and on the mechanical properties. This RMGIC and CHX combination completely eliminated mutans streptococci after 3 months of IPT. Conclusion: The RMGIC and 1.25% CHX mixture showed great biological and mechanical behaviour and could be a good treatment against caries progression. Clinical significance: The association of CHX with a liner RMGIC opens a new perspective for arresting residual caries after IPT. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/00359-0 - Evaluation of biological and physico-mechanical properties of glass ionomer cements associated to chlorhexidine or doxycycline as liner material in procedures of partial caries removal
Grantee:Aline Rogéria Freire de Castilho
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 08/02606-5 - Evaluation of biological and physico-mechanical properties of glass ionomer cements associated to chlorhexidine or doxycycline as liner material in procedures of partial caries removal
Grantee:Regina Maria Puppin Rontani
Support type: Regular Research Grants