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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure

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Autor(es):
Lanhellas Goncalves, Frances Lilian [1] ; Gallindo, Rodrigo Melo [1] ; Mangueira Soares, Lucas Manoel [1] ; Figueira, Rebeca Lopes [1] ; Perecim Volpe, Fabio Antonio [1] ; Pereira-da-Silva, Maurcio Andre [1] ; Sbragia, Lourenco [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo FMRP USP, Sch Med Ribeirao Preto, Dept Surg & Anat, Div Pediat Surg, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Acta Cirurgica Brasileira; v. 28, n. 1, p. 19-25, 2013.
Citações Web of Science: 2
Resumo

PURPOSE: To describe the difficulties of implementing the protocol of experimental necrotizing enterocolitis (NEC) in order to obtain a larger number of newborns affected with the disease and a lower mortality. METHODS: Term Sprague-Dawley newborns rats (22 days) were divided into four groups of 12 fetuses each (n = 48): EC - breastfed newborns; IH - breastfed newborns and subjected to a stress protocol by ischemia and hypothermia; ESB - formula-fed newborns (Esbilac (R), PetAg, Hampshire, IL, USA) and NEC - formula-fed newborns and subjected to stress protocol. The parameters set for the study protocol were: milk concentration (0.19 g ml or 0.34 g/ml), diet instilled volume (according to body weight - 200 kcal/day/Kg - or progressive, according to acceptance), weight (gain, loss or maintenance) and duration of the experiment (72 hours or 96 hours). Data of body weight (BW), intestinal weight (IW) and the IW/BW ratio were obtained. Samples of terminal ileum were collected and analyzed by the degree of injury to the intestinal wall. Statistically significance was set to p<0.05. RESULTS: The established protocol with less mortality and increased number of NEC was with Esbilac (R) at a concentration of 0.19 g/ml of diet instilled volume of 0.1 ml, every 3 hours, for 72 hours. All infants fed with artificial milk lost weight. In the degree score of intestinal injury, the ESB, IH and NEC groups were considered positive for NEC with greater histological injury in the latter. CONCLUSION: The described NEC protocol in rats allowed a greater survival of puppies with a greater number of animals affected by the disease. (AU)

Processo FAPESP: 11/12587-0 - Avaliação in vivo do efeito do corticóide e da ventilação na via do óxido nítrico sobre a expressão do VEGF e seus receptores pulmonares após a oclusão traqueal fetal na hérnia diafragmática congênita em ratos
Beneficiário:Frances Lilian Lanhellas Gonçalves
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/00794-1 - Avaliação da via do óxido nítrico in vivo após a oclusão traqueal fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática congênita em ratos induzida pelo Nitrofen
Beneficiário:Lourenço Sbragia Neto
Linha de fomento: Auxílio à Pesquisa - Regular