Performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples

Autor(es):Mostrar menos -

LRA Vaz-de-Lima ^[1] ; MCO Souza ^[2] ; TK Matsumoto ^[3] ; MA Hong ^[4] ; MM Salgado ^[5] ; ML Barbosa ^[6] ; NS Sato ^[7] ; HI Requejo ^[8] ; CAF Oliveira ^[9] ; R Pecchini ^[10] ; E Berezin ^[11] ; SD Passos ^[12] ; C Schvartsman ^[13] ; A Pasmanick ^[14] ; EL Durigon ; M Ueda ^[16]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[2] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[3] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[4] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[5] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[6] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[7] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[8] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[9] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

^[10] Irmandade Santa Casa de Misericórdia de São Paulo - Brasil

^[11] Irmandade Santa Casa de Misericórdia de São Paulo - Brasil

^[12] Faculdade de Medicina de Jundiaí. Hospital Universitário - Brasil

^[13] Faculdade de Medicina. Instituto da Criança

^[14] Faculdade de Medicina. Instituto da Criança

^[16] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 103, n. 5, p. 463-467, 2008-08-00.

Resumo

Comparison of the use of indirect immunofluorescence assay (IFA), immunochromatography assay (ICA-BD) and reverse transcription-polymerase chain reaction (RT-PCR) for detecting human respiratory syncytial virus (HRSV) in 306 nasopharyngeal aspirates samples (NPA) was performed in order to assess their analytical performance. By comparing the results obtained using ICA-BD with those using IFA, we found relative indices of 85.0% for sensitivity and 91.2% for specificity, and the positive (PPV) and negative (NPV) predictive values were 85.0% and 91.2%, respectively. The relative indices for sensitivity and specificity as well as the PPV and NPV for RT-PCR were 98.0%, 89.0%, 84.0% and 99.0%, respectively, when compared to the results of IFA. In addition, comparison of the results of ICA-BD and those of RT-PCR yielded relative indices of 79.5% for sensitivity and 95.4% for specificity, as well as PPV and NPV of 92.9% and 86.0%, respectively. Although RT-PCR has shown the best performance, the substantial agreement between the ICA-BD and IFA results suggests that ICA-BD, also in addition to being a rapid and facile assay, could be suitable as an alternative diagnostic screening for HRSV infection in children. (AU)

• Processo FAPESP: 02/08462-9 - Rede de Diversidade Genética de Vírus (RDGV)
• Beneficiário: Mirthes Ueda
• Modalidade de apoio: Auxílio à Pesquisa - Regular