Modeling the Trypanosoma cruzi Tc85-11 protein and mapping the laminin-binding site

Autor(es)

Marroquin-Quelopana, Miryam ; Oyama Júnior, Sergio ; Pertinhez, Thelma Aguiar ; Spisni, Alberto ; Juliano, Maria Aparecida ; Juliano, Luiz ; Colli, Walter ; Alves, Maria Júlia M. ^[8]

• Tipo de documento: Artigo Científico
• Fonte: Biochemical and Biophysical Research Communications; v. 325, n. 2, p. 612-618, Dec. 2004.
• Área do conhecimento: Ciências Biológicas - Bioquímica
• Assunto(s): Protozoa Trypanosoma cruzi Estrutura terciária de proteína Laminina

Resumo

Trypanosoma cruzi expresses a set of glycoproteins encoded by the gp85/trans-sialidase gene superfamily. In this report a structure model is proposed for a cloned member of the superfamily, the Tc85-11 protein. The structure consists of an N-terminus â-propeller and a C-terminus â-sandwich interconnected by an á-helix. The recombinant protein, corresponding to the N-domain (Tc85-N), binds to laminin in a selective manner. Six synthetic 20-mer peptides from the N-domain adhere onto the surface of LLC-MK2 cells and two of these peptides specifically inhibit the Tc85-N/laminin interaction, indicating that they are the laminin-binding sites of the molecule. Thus, Tc85-11 and other related members of the family appear to be good candidates to play an important role in T. cruzi infection via a laminin mediated host-parasite interaction. (AU)

• Processo FAPESP: 00/02026-7 - Correlação estrutura-função de proteínas e peptídeos
• Beneficiário: Thelma de Aguiar Pertinhez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 01/08001-9 - Modelagem molecular e biologia estrutural.
• Beneficiário: Sergio Oyama Junior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 99/12459-9 - Trypanosoma cruzi: interação parasita-célula hospedeira
• Beneficiário: Maria Julia Manso Alves
• Modalidade de apoio: Auxílio à Pesquisa - Temático