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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cellular prion protein transduces neuroprotective signals

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Autor(es):
Chiarini, Luciana B. ; Freitas, Adriana R. O. ; Zanata, Silvio M. ; Brentani, Ricardo R. ; Martins, Vilma R. [5] ; Linden, Rafael
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: EMBO Journal; v. 21, n. 13, p. 3317-3326, July 2002.
Área do conhecimento: Ciências Biológicas - Bioquímica
Assunto(s):Transdução de sinais   Morte celular   Apoptose   Neurônios   Proteínas   Príons
Resumo

To test for a role for the cellular prion protein (PrPc) in cell death, we used a PrPc-binding peptide. Retinal explants from neonatal rats or mice were kept in vitro for 24 h, and anisomycin (ANI) was used to induce apoptosis. The peptide activated both cAMP/protein kinase A (PKA) and Erk pathways, and partially prevented cell death induced by ANI in explants from wild-type rodents, but not from PrPc-null mice. Neuroprotection was abolished by treatment with phosphatidylinositol-specific phospholipase C, with human peptide 106-126, with certain antibodies to PrPc or with a PKA inhibitor, but not with a MEK/Erk inhibitor. In contrast, antibodies to PrPc that increased cAMP also induced neuroprotection. Thus, engagement of PrPc transduces neuroprotective signals through a cAMP/PKA-dependent pathway. PrPc may function as a trophic receptor, the activation of which leads to a neuroprotective state. (AU)

Processo FAPESP: 99/07124-8 - Papel da proteína prion celular em processos fisiológicos e patológicos
Beneficiário:Vilma Regina Martins
Linha de fomento: Auxílio à Pesquisa - Temático