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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cellular prion protein interaction with vitronectin supports axonal growth and is compensated by integrins

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Hajj, Glaucia N. M. ; Lopes, Marilene H. ; Mercadante, Adriana F. ; Veiga, Silvio S. ; Silveira, Rafael B. da ; Santos, Tiago G. ; Ribeiro, Karina C. B. ; Juliano, Maria A. ; Jacchieri, Saul G. ; Zanata, Silvio M. ; Martins, Vilma R. [11]
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Journal of Cell Science; v. 120, n. 11, p. 1915-1926, May 2007.
Área do conhecimento: Ciências Biológicas - Bioquímica
Assunto(s):Gânglios espinhais   Matriz extracelular   Proteínas   Príons   Vitronectina   Integrinas   Neurônios   Axônios
Resumo

The physiological functions of the cellular prion protein, PrPC, as a cell surface pleiotropic receptor are under debate. We report that PrPC interacts with vitronectin but not with fibronectin or collagen. The binding sites mediating this PrPC-vitronectin interaction were mapped to residues 105-119 of PrPC and the residues 307-320 of vitronectin. The two proteins were co-localized in embryonic dorsal root ganglia from wild-type mice. Vitronectin addition to cultured dorsal root ganglia induced axonal growth, which could be mimicked by vitronectin peptide 307-320 and abrogated by anti-PrPC antibodies. Full-length vitronectin, but not the vitronectin peptide 307-320, induced axonal growth of dorsal root neurons from two strains of PrPC-null mice. Functional assays demonstrated that relative to wild-type cells, PrPCnull dorsal root neurons were more responsive to the Arg- Gly-Asp peptide (an integrin-binding site), and exhibited greater ALPHA-v-BETA-3 activity. Our findings indicate that PrPC plays an important role in axonal growth, and this function may be rescued in PrPC-knockout animals by integrin compensatory mechanisms. (AU)

Processo FAPESP: 99/07124-8 - Papel da proteína prion celular em processos fisiológicos e patológicos
Beneficiário:Vilma Regina Martins
Linha de fomento: Auxílio à Pesquisa - Temático