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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cellular prion protein interaction with vitronectin supports axonal growth and is compensated by integrins

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Hajj, Glaucia N. M. ; Lopes, Marilene H. ; Mercadante, Adriana F. ; Veiga, Silvio S. ; Silveira, Rafael B. da ; Santos, Tiago G. ; Ribeiro, Karina C. B. ; Juliano, Maria A. ; Jacchieri, Saul G. ; Zanata, Silvio M. ; Martins, Vilma R. [11]
Total Authors: 11
Document type: Journal article
Source: Journal of Cell Science; v. 120, n. 11, p. 1915-1926, May 2007.
Field of knowledge: Biological Sciences - Biochemistry
Abstract

The physiological functions of the cellular prion protein, PrPC, as a cell surface pleiotropic receptor are under debate. We report that PrPC interacts with vitronectin but not with fibronectin or collagen. The binding sites mediating this PrPC-vitronectin interaction were mapped to residues 105-119 of PrPC and the residues 307-320 of vitronectin. The two proteins were co-localized in embryonic dorsal root ganglia from wild-type mice. Vitronectin addition to cultured dorsal root ganglia induced axonal growth, which could be mimicked by vitronectin peptide 307-320 and abrogated by anti-PrPC antibodies. Full-length vitronectin, but not the vitronectin peptide 307-320, induced axonal growth of dorsal root neurons from two strains of PrPC-null mice. Functional assays demonstrated that relative to wild-type cells, PrPCnull dorsal root neurons were more responsive to the Arg- Gly-Asp peptide (an integrin-binding site), and exhibited greater ALPHA-v-BETA-3 activity. Our findings indicate that PrPC plays an important role in axonal growth, and this function may be rescued in PrPC-knockout animals by integrin compensatory mechanisms. (AU)

FAPESP's process: 99/07124-8 - The role of celular prion protein in physiological and pathological processes
Grantee:Vilma Regina Martins
Support type: Research Projects - Thematic Grants