Phenotypic Susceptibility to Antiretrovirals Among Clades C, F, and B/F Recombinant Antiretroviral-Naive HIV Type 1 Strains

Autor(es)

Sucupira, Maria Cecilia A. ^[1] ; Munerato, Patricia ^{[1, 2]} ; Silveira, Jussara ^[3] ; Santos, Andre F. ^[4] ; Janini, Luiz Mario ^[1] ; Soares, Marcelo A. ^{[4, 5]} ; Diaz, Ricardo Sobhie ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, BR-04039032 Sao Paulo - Brazil

^[2] Life Technol Com & Ind Prod Brasil, Sao Paulo - Brazil

^[3] Fundacao Univ Fed Rio Grande, Rio Grande - Brazil

^[4] Univ Fed Rio de Janeiro, Rio De Janeiro - Brazil

^[5] Inst Nacl Canc, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AIDS Research and Human Retroviruses; v. 29, n. 6, p. 880-886, JUN 2013.
• Citações Web of Science: 3

Resumo

To evaluate antiretroviral phenotypic susceptibility of wild-type HIV-1 strains circulating in Brazil, samples from antiretroviral-naive individuals infected with subtypes C (n = 16), F (n = 9), or B/F (n = 7), where reverse transcriptase is B and protease is F, were phenotyped using the Antivirogram Assay (Virco, Mechelen, Belgium). Reduced susceptibility to protease inhibitors (PIs) was observed in one C and three F isolates. None of these samples had any known PI resistance mutations. The phenotypic fold change to one PI was above the biological cut-off in three of 96 (3.1%) clade F phenotypic determinations and in one of 96 (1.0%) clade C. Phenotypic resistance to at least one nucleoside reverse transcriptase inhibitor (NRTI) was found for two B/F, four C, and three F isolates. The phenotypic fold change in susceptibility to NRTIs was above the cut-off value in nine of 111 (8.1%) clade C determinations, as compared to three of 63 (4.8%) for clade F and two of 49 (4.1%) for clade B. The phenotypic fold change to non-NRTI (NNRTI) was above the cut-off in seven of 32 (21.9%) of C isolates determinations, whereas none of the F isolates had a decrease of susceptibility. Only two of the 16 C samples had a known NNRTI resistance mutation. The NNRTI fold change was above the cut-off value in three of 14 (21.4%) phenotypic determinations of Brazilian B/F recombinants, representing clade B reverse transcriptase. NNRTI susceptibility should be better investigated in clade C and B/F recombinants. (AU)

• Processo FAPESP: 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Temático