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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phenotypic Susceptibility to Antiretrovirals Among Clades C, F, and B/F Recombinant Antiretroviral-Naive HIV Type 1 Strains

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Author(s):
Sucupira, Maria Cecilia A. [1] ; Munerato, Patricia [1, 2] ; Silveira, Jussara [3] ; Santos, Andre F. [4] ; Janini, Luiz Mario [1] ; Soares, Marcelo A. [4, 5] ; Diaz, Ricardo Sobhie [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, BR-04039032 Sao Paulo - Brazil
[2] Life Technol Com & Ind Prod Brasil, Sao Paulo - Brazil
[3] Fundacao Univ Fed Rio Grande, Rio Grande - Brazil
[4] Univ Fed Rio de Janeiro, Rio De Janeiro - Brazil
[5] Inst Nacl Canc, Rio De Janeiro - Brazil
Total Affiliations: 5
Document type: Journal article
Source: AIDS Research and Human Retroviruses; v. 29, n. 6, p. 880-886, JUN 2013.
Web of Science Citations: 3
Abstract

To evaluate antiretroviral phenotypic susceptibility of wild-type HIV-1 strains circulating in Brazil, samples from antiretroviral-naive individuals infected with subtypes C (n = 16), F (n = 9), or B/F (n = 7), where reverse transcriptase is B and protease is F, were phenotyped using the Antivirogram Assay (Virco, Mechelen, Belgium). Reduced susceptibility to protease inhibitors (PIs) was observed in one C and three F isolates. None of these samples had any known PI resistance mutations. The phenotypic fold change to one PI was above the biological cut-off in three of 96 (3.1%) clade F phenotypic determinations and in one of 96 (1.0%) clade C. Phenotypic resistance to at least one nucleoside reverse transcriptase inhibitor (NRTI) was found for two B/F, four C, and three F isolates. The phenotypic fold change in susceptibility to NRTIs was above the cut-off value in nine of 111 (8.1%) clade C determinations, as compared to three of 63 (4.8%) for clade F and two of 49 (4.1%) for clade B. The phenotypic fold change to non-NRTI (NNRTI) was above the cut-off in seven of 32 (21.9%) of C isolates determinations, whereas none of the F isolates had a decrease of susceptibility. Only two of the 16 C samples had a known NNRTI resistance mutation. The NNRTI fold change was above the cut-off value in three of 14 (21.4%) phenotypic determinations of Brazilian B/F recombinants, representing clade B reverse transcriptase. NNRTI susceptibility should be better investigated in clade C and B/F recombinants. (AU)

FAPESP's process: 04/15856-9 - Prospective analysis of the virological and immunological characteristics in individuals with recent HIV-1 infection in the cities of São Paulo and Santos
Grantee:Ricardo Sobhie Diaz
Support Opportunities: Research Projects - Thematic Grants