Changes in gene expression in the frontal cortex of rats with pilocarpine-induced status epilepticus after sleep deprivation

Autor(es)

Hirotsu, Camila ^[1] ; Matos, Gabriela ^[1] ; Tufik, Sergio ^[1] ; Andersen, Monica Levy ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psicobiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Epilepsy & Behavior; v. 27, n. 2, p. 378-384, MAY 2013.
• Citações Web of Science: 9

Resumo

Sleep and epilepsy present a bidirectional interaction. Sleep complaints are common in epilepsy, and sleep deprivation may provoke seizures. However, the mechanisms underlying this relationship are unknown. Thus, this study investigated the effects of paradoxical sleep deprivation (PSD24h) and total sleep deprivation (TSD6h) in the expression of genes related to reactive oxygen species and nitric oxide production in the frontal cortex of a rodent model of temporal lobe epilepsy (PILO). The data show that PILO rats had increased NOX-2 expression and decreased SOD expression, independent of sleep. Higher NOX-2 expression was observed only in PILO rats subjected to the control condition and TSD6h. Also, eNOS and DDAH1 were increased in the PILO group submitted to TSD6h. Moreover, CAT expression in the frontal cortex of PILO rats submitted to PSD24h was reduced compared to that of PILO rats that were not sleep-deprived. The molecular changes found in the frontal cortex of PILO rats following sleep deprivation suggest a mechanism via oxidative stress. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/05396-7 - Efeitos no desenvolvimento sensorial e sexual da prole de ratas submetidas à restrição de sono
• Beneficiário: Monica Levy Andersen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/15110-8 - Sono e morte súbita nas epilepsias: provável base molecular?
• Beneficiário: Gabriela de Matos Barbosa Pimenta
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/50129-1 - Insuficiência renal crônica, restrição de sono e sildenafil: consequências renais, cardíacas e sexuais em um modelo animal
• Beneficiário: Camila Hirotsu
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto