Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation

Autor(es)

Hatanaka, Elaine ^{[1, 2]} ; Dermargos, Alexandre ^{[3, 4]} ; Hirata, Aparecida Emiko ^[5] ; Ramirez Vinolo, Marco Aurelio ^{[1, 6]} ; Carpinelli, Angelo Rafael ^[1] ; Newsholme, Philip ^{[7, 8]} ; Armelin, Hugo Aguirre ^{[3, 4]} ; Curi, Rui ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil

^[2] Cruzeiro do Sul Univ, Inst Phys Act & Sport Sci, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Quim, Sao Paulo - Brazil

^[4] Inst Butantan, Lab Especial Ciclo Celular, Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Dept Fisiol, Sao Paulo - Brazil

^[6] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Sao Paulo - Brazil

^[7] Univ Coll Dublin, Conway Inst, Sch Biomol & Biomed Sci, Dublin 2 - Ireland

^[8] Curtin Univ, Sch Biomed Sci, Perth, WA - Australia

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 8, n. 4 APR 8 2013.
• Citações Web of Science: 20

Resumo

The effect of oleic, linoleic and gamma-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and gamma-linolenic acids. ROS production was dependent on the addition of beta-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and gamma-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47(phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47(phox) mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: gamma-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and gamma-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts. (AU)

• Processo FAPESP: 04/12466-5 - Funcao dos acidos oleico, linoleico e a-linolenico nos mecanismos envolvidos no processo de cicatrizacao.
• Beneficiário: Elaine Hatanaka Dermargos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/51273-9 - Dualidade funcional do FGF2 (Fibroblast Growth Factor 2) no controle do ciclo celular: mitógeno e supressor de tumor
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/52043-1 - Funcao dos acidos oleico,linoleico e gama-linoleico nos mecanismos envolvidos no processod e cicatrizacao.
• Beneficiário: Rui Curi
• Modalidade de apoio: Auxílio à Pesquisa - Regular