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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation

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Hatanaka, Elaine [1, 2] ; Dermargos, Alexandre [3, 4] ; Hirata, Aparecida Emiko [5] ; Ramirez Vinolo, Marco Aurelio [2, 6] ; Carpinelli, Angelo Rafael [2] ; Newsholme, Philip [7, 8] ; Armelin, Hugo Aguirre [3, 4] ; Curi, Rui [2]
Total Authors: 8
[1] Cruzeiro do Sul Univ, Inst Phys Act & Sport Sci, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim, Sao Paulo - Brazil
[4] Inst Butantan, Lab Especial Ciclo Celular, Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Fisiol, Sao Paulo - Brazil
[6] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Sao Paulo - Brazil
[7] Univ Coll Dublin, Conway Inst, Sch Biomol & Biomed Sci, Dublin 2 - Ireland
[8] Curtin Univ, Sch Biomed Sci, Perth, WA - Australia
Total Affiliations: 8
Document type: Journal article
Source: PLoS One; v. 8, n. 4 APR 8 2013.
Web of Science Citations: 20

The effect of oleic, linoleic and gamma-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and gamma-linolenic acids. ROS production was dependent on the addition of beta-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and gamma-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47(phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47(phox) mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: gamma-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and gamma-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts. (AU)

FAPESP's process: 08/51273-9 - FGF2 dual function in cell cycle control: mitogen and tumor suppressor
Grantee:Hugo Aguirre Armelin
Support type: Research Projects - Thematic Grants