| Full text | |
| Author(s): |
Hatanaka, Elaine
[1, 2]
;
Dermargos, Alexandre
[3, 4]
;
Hirata, Aparecida Emiko
[5]
;
Ramirez Vinolo, Marco Aurelio
[1, 6]
;
Carpinelli, Angelo Rafael
[1]
;
Newsholme, Philip
[7, 8]
;
Armelin, Hugo Aguirre
[3, 4]
;
Curi, Rui
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[2] Cruzeiro do Sul Univ, Inst Phys Act & Sport Sci, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim, Sao Paulo - Brazil
[4] Inst Butantan, Lab Especial Ciclo Celular, Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Fisiol, Sao Paulo - Brazil
[6] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Sao Paulo - Brazil
[7] Univ Coll Dublin, Conway Inst, Sch Biomol & Biomed Sci, Dublin 2 - Ireland
[8] Curtin Univ, Sch Biomed Sci, Perth, WA - Australia
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | PLoS One; v. 8, n. 4 APR 8 2013. |
| Web of Science Citations: | 20 |
| Abstract | |
The effect of oleic, linoleic and gamma-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and gamma-linolenic acids. ROS production was dependent on the addition of beta-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and gamma-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47(phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47(phox) mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: gamma-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and gamma-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts. (AU) | |
| FAPESP's process: | 08/51273-9 - FGF2 dual function in cell cycle control: mitogen and tumor suppressor |
| Grantee: | Hugo Aguirre Armelin |
| Support Opportunities: | Research Projects - Thematic Grants |