Apoptosis through Bcl-2/Bax and Cleaved Caspase Up-Regulation in Melanoma Treated by Boron Neutron Capture Therapy

Autor(es)

Faiao-Flores, Fernanda ^{[1, 2]} ; Pinto Coelho, Paulo Rogerio ^[3] ; Toledo Arruda-Neto, Joao Dias ^{[4, 5]} ; Maria-Engler, Silvya Stuchi ^[6] ; Tiago, Manoela ^[6] ; Capelozzi, Vera Luiza ^[1] ; Giorgi, Ricardo Rodrigues ^{[7, 8]} ; Maria, Durvanei Augusto ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Sao Paulo - Brazil

^[2] Butantan Inst, Biochem & Biophys Lab, Sao Paulo - Brazil

^[3] Inst Nucl & Energy Res, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Phys, Sao Paulo - Brazil

^[5] CEPESq Unitalo Italy Brazilian Univ Ctr, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Dept Clin Chem & Toxicol, Sch Pharmaceut Sci, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Sch Med, Lab Cellular & Mol Endocrinol LIM 25, Sao Paulo - Brazil

^[8] Santo Amaro Univ UNISA, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 8, n. 3 MAR 20 2013.
• Citações Web of Science: 16

Resumo

Boron neutron capture therapy (BNCT) is a binary treatment involving selective accumulation of boron carriers in a tumor followed by irradiation with a thermal or epithermal neutron beam. The neutron capture reaction with a boron-10 nucleus yields high linear energy transfer (LET) particles, alpha and Li-7, with a range of 5 to 9 mu m. These particles can only travel very short distances and release their damaging energy directly into the cells containing the boron compound. We aimed to evaluate proliferation, apoptosis and extracellular matrix (ECM) modifications of B16F10 melanoma and normal human melanocytes after BNCT. The amounts of soluble collagen and Hsp47, indicating collagen synthesis in the ECM, as well as the cellular markers of apoptosis, were investigated. BNCT decreased proliferation, altered the ECM by decreasing collagen synthesis and induced apoptosis by regulating Bcl-2/Bax in melanoma. Additionally, BNCT also increased the levels of TNF receptor and the cleaved caspases 3, 7, 8 and 9 in melanoma. These results suggest that multiple pathways related to cell death and cell cycle arrest are involved in the treatment of melanoma by BNCT. (AU)

• Processo FAPESP: 08/56397-8 - Avaliação da atividade antitumoral do composto sintético DM-1 e terapia de captação de nêutrons por boro (BNCT) associados ao quimioterápico dacarbazina no tratamento do melanoma
• Beneficiário: Fernanda Faião Flores
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 08/58817-4 - Geração de peles artificiais humanas e melanomas invasivos como plataforma para testes farmacológicos
• Beneficiário: Silvya Stuchi Maria-Engler
• Linha de fomento: Auxílio à Pesquisa - Regular