In vitro anti-allergic activity of the fungal metabolite pyridovericin

Autor(es)

Santos, Marcela de Souza ^{[1, 2]} ; Andrioli, Willian Jonis ^[3] ; Freire de Morais Del Lama, Maria Perpetua ^{[1, 2]} ; Bastos, Jairo Kenupp ^[3] ; Dhammika Nanayakkara, N. P. ^[4] ; Zumstein Georgetto Naal, Rose Mary ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, BR-14040903 Sao Paulo - Brazil

^[2] Inst Natl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil

^[3] Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 Sao Paulo - Brazil

^[4] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 - USA

• Tipo de documento: Artigo Científico
• Fonte: International Immunopharmacology; v. 15, n. 3, p. 532-538, MAR 2013.
• Citações Web of Science: 7

Resumo

Mast cells play a critical role during the development of an allergic response. Upon activation by an antigen and IgE, via Fc epsilon RI receptors, mast cells release histamine and other mediators that initiate and propagate immediate hypersensitivity reactions. Mast cells also secrete cytokines that regulate the immune responses. In this way, inhibitors of mast cell activity could work as promising therapeutics for allergic disorders. In the present work, we investigated the capacity of pyridovericin, a natural product isolated from the entomopathogenic fungus Beauveria bassiana, to inhibit mast cell degranulation and cytokine secretion. It was found that pyridovericin strongly decreased the release of beta-hexosaminidase, a marker for mast cell degranulation, when mast cells were stimulated by both Fc epsilon RI-dependent and independent pathways. In addition, pyridovericin strongly abrogated secretion of interleukin-4. Pyridovericin-mediated suppression of stimulated increase in intracellular Ca2+ levels, a crucial signal for mounting of both degranulation and cytokine production responses, was ascribed as one of the inhibition targets of pyridovericin. Those initial studies identify pyridovericin as a potential new candidate for the development of new anti-allergic drugs. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/01712-6 - Estudo da atividade antialérgica de cumarinas naturais e sintéticas: aplicação do biossensor baseado em mastócitos
• Beneficiário: Marcela de Souza Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto