In vitro anti-allergic activity of the fungal metabolite pyridovericin

Author(s)

Santos, Marcela de Souza ^{[1, 2]} ; Andrioli, Willian Jonis ^[3] ; Freire de Morais Del Lama, Maria Perpetua ^{[1, 2]} ; Bastos, Jairo Kenupp ^[3] ; Dhammika Nanayakkara, N. P. ^[4] ; Zumstein Georgetto Naal, Rose Mary ^{[1, 2]}

Affiliation

^[1] Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, BR-14040903 Sao Paulo - Brazil

^[2] Inst Natl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil

^[3] Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 Sao Paulo - Brazil

^[4] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 - USA

• Document type: Journal article
• Source: International Immunopharmacology; v. 15, n. 3, p. 532-538, MAR 2013.
• Web of Science Citations: 7

Abstract

Mast cells play a critical role during the development of an allergic response. Upon activation by an antigen and IgE, via Fc epsilon RI receptors, mast cells release histamine and other mediators that initiate and propagate immediate hypersensitivity reactions. Mast cells also secrete cytokines that regulate the immune responses. In this way, inhibitors of mast cell activity could work as promising therapeutics for allergic disorders. In the present work, we investigated the capacity of pyridovericin, a natural product isolated from the entomopathogenic fungus Beauveria bassiana, to inhibit mast cell degranulation and cytokine secretion. It was found that pyridovericin strongly decreased the release of beta-hexosaminidase, a marker for mast cell degranulation, when mast cells were stimulated by both Fc epsilon RI-dependent and independent pathways. In addition, pyridovericin strongly abrogated secretion of interleukin-4. Pyridovericin-mediated suppression of stimulated increase in intracellular Ca2+ levels, a crucial signal for mounting of both degranulation and cytokine production responses, was ascribed as one of the inhibition targets of pyridovericin. Those initial studies identify pyridovericin as a potential new candidate for the development of new anti-allergic drugs. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 08/01712-6 - Study of antialergic activity of natural and synthetic coumarins: application of a biosensor based on mast cells
• Grantee: Marcela de Souza Santos
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)