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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

FISH analysis for TET2 deletion in a cohort of 362 Brazilian myeloid malignancies: correlation with karyotype abnormalities

Texto completo
Autor(es):
de Oliveira, Fabio Morato [1, 2] ; Miguel, Carlos Eduardo [3] ; Lucena-Araujo, Antonio Roberto [1, 2] ; Gouvea de Lima, Ana Silvia [1, 2] ; Falcao, Roberto Passetto [1, 2] ; Rego, Eduardo Magalhaes [1, 2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Dept Internal Med, Div Hematol, Sch Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Hosp Base Sao Jose do Rio Preto, Div Hematol, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: MEDICAL ONCOLOGY; v. 30, n. 1 MAR 2013.
Citações Web of Science: 2
Resumo

We investigated the prevalence of TET2 deletion by using a new FISH probe in a cohort of 362 Brazilian patients with myeloid neoplasms and their association with cytogenetic information (G-banding analysis). Normal karyotype was observed in 45.8 % of MDS(n = 44), 43.8 % of AML (n = 39) and 46.3 % of MPN (n = 82). Abnormalities of 4q24 (deletions, translocations or inversions) were associated with another chromosomal abnormality in four patients by G-banding analysis (2 MDS, 1 AML and 1 MPN). Interphase FISH analysis revealed deletion of TET2 in 21 patients (6 patients with abnormal karyotype and in 15 patients with normal karyotype). arrayCGH analysis revealed a cryptic deletion of the region 4q24 in all eight patients selected with myeloid malignancies (3 MDS, 1 AML and 4 MPN). Considering the significantly high cost of determining the mutational status of TET2 in patient samples by using conventional sequencing methods and sometimes the lack of regular use of SNP/aCGH array methodologies, FISH for the detection of TET2 abnormalities may become a potentially useful clinical tool. The search for alterations in TET2 gene may be important for the prediction of prognosis in normal/altered AML patients' karyotype or in the disease evolution of patients with MNP and MDS. (AU)

Processo FAPESP: 11/01647-2 - Avaliação da instabilidade genômica, por meio da organização tridimensional nuclear de telômeros, na sindrome mielodisplásica (SMD)
Beneficiário:Fábio Morato de Oliveira
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores