CD57 Expression and Cytokine Production by T Cells in Lesional and Unaffected Skin from Patients with Psoriasis

Autor(es):Mostrar menos -

Batista, Mariana D. ^{[1, 2]} ; Tincati, Camilla ^[2] ; Milush, Jeffrey M. ^[2] ; Ho, Emily L. ^[2] ; Ndhlovu, Lishomwa C. ^[2] ; York, Vanessa A. ^[2] ; Kallas, Esper G. ^{[1, 3]} ; Kalil, Jorge ^{[1, 3]} ; Keating, Sheila M. ^{[4, 5]} ; Norris, Philip J. ^{[4, 6, 5]} ; Chang, David ^[7] ; Unemori, Patrick ^[8] ; Leslie, Kieron S. ^[8] ; Maurer, Toby ^[8] ; Liao, Wilson ^[8] ; Nixon, Douglas F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Div Clin Immunol & Allergy, Sao Paulo - Brazil

^[2] Univ Calif San Francisco, Dept Med, Div Expt Med, San Francisco, CA 94143 - USA

^[3] Univ Sao Paulo, Inst Invest Imunol, Sao Paulo - Brazil

^[4] Blood Syst Res Inst, San Francisco, CA - USA

^[5] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA - USA

^[6] Univ Calif San Francisco, Dept Med, San Francisco, CA - USA

^[7] Calif Pacific Med Ctr, San Francisco, CA - USA

^[8] Univ Calif San Francisco, Dept Dermatol, San Francisco, CA 94143 - USA

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 8, n. 2 FEB 28 2013.
• Citações Web of Science: 3

Resumo

Background: The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions. Methodology: We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients. Principal Findings: We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin. Conclusions/Significance: These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover. (AU)

• Processo FAPESP: 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/05845-0 - Resposta imune celular em doenças infecciosas e imunodeficiências primárias
• Beneficiário: Esper Georges Kallás
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional