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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

CD57 Expression and Cytokine Production by T Cells in Lesional and Unaffected Skin from Patients with Psoriasis

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Author(s):
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Batista, Mariana D. [1, 2] ; Tincati, Camilla [2] ; Milush, Jeffrey M. [2] ; Ho, Emily L. [2] ; Ndhlovu, Lishomwa C. [2] ; York, Vanessa A. [2] ; Kallas, Esper G. [1, 3] ; Kalil, Jorge [1, 3] ; Keating, Sheila M. [4, 5] ; Norris, Philip J. [4, 6, 5] ; Chang, David [7] ; Unemori, Patrick [8] ; Leslie, Kieron S. [8] ; Maurer, Toby [8] ; Liao, Wilson [8] ; Nixon, Douglas F. [2]
Total Authors: 16
Affiliation:
[1] Univ Sao Paulo, Sch Med, Div Clin Immunol & Allergy, Sao Paulo - Brazil
[2] Univ Calif San Francisco, Dept Med, Div Expt Med, San Francisco, CA 94143 - USA
[3] Univ Sao Paulo, Inst Invest Imunol, Sao Paulo - Brazil
[4] Blood Syst Res Inst, San Francisco, CA - USA
[5] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA - USA
[6] Univ Calif San Francisco, Dept Med, San Francisco, CA - USA
[7] Calif Pacific Med Ctr, San Francisco, CA - USA
[8] Univ Calif San Francisco, Dept Dermatol, San Francisco, CA 94143 - USA
Total Affiliations: 8
Document type: Journal article
Source: PLoS One; v. 8, n. 2 FEB 28 2013.
Web of Science Citations: 3
Abstract

Background: The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions. Methodology: We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients. Principal Findings: We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin. Conclusions/Significance: These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover. (AU)

FAPESP's process: 04/15856-9 - Prospective analysis of the virological and immunological characteristics in individuals with recent HIV-1 infection in the cities of São Paulo and Santos
Grantee:Ricardo Sobhie Diaz
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 10/05845-0 - Cellular immune responses in infectious diseases and primary immunodeficiencies
Grantee:Esper Georges Kallás
Support Opportunities: Research Grants - Visiting Researcher Grant - International