Nanobiosensors Based on Chemically Modified AFM Probes: A Useful Tool for Metsulfuron-Methyl Detection

Autor(es)

da Silva, Aline C. N. ^[1] ; Deda, Daiana K. ^[1] ; da Roz, Alessandra L. ^[1] ; Prado, Rogilene A. ^[2] ; Carvalho, Camila C. ^[2] ; Viviani, Vadim ^[2] ; Leite, Fabio L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Phys Chem & Math, Multidisciplinary Lab Nanoneurobiophys, Nanoneurobiophys Res Grp, BR-18052780 Sorocaba, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Phys Chem & Math, Lab Biochem & Biotechnol Bioluminescence, BR-18052780 Sorocaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SENSORS; v. 13, n. 2, p. 1477-1489, FEB 2013.
• Citações Web of Science: 22

Resumo

The use of agrochemicals has increased considerably in recent years, and consequently, there has been increased exposure of ecosystems and human populations to these highly toxic compounds. The study and development of methodologies to detect these substances with greater sensitivity has become extremely relevant. This article describes, for the first time, the use of atomic force spectroscopy (AFS) in the detection of enzyme-inhibiting herbicides. A nanobiosensor based on an atomic force microscopy (AFM) tip functionalised with the acetolactate synthase (ALS) enzyme was developed and characterised. The herbicide metsulfuron-methyl, an ALS inhibitor, was successfully detected through the acquisition of force curves using this biosensor. The adhesion force values were considerably higher when the biosensor was used. An increase of similar to 250% was achieved relative to the adhesion force using an unfunctionalised AFM tip. This considerable increase was the result of a specific interaction between the enzyme and the herbicide, which was primarily responsible for the efficiency of the nanobiosensor. These results indicate that this methodology is promising for the detection of herbicides, pesticides, and other environmental contaminants. (AU)

• Processo FAPESP: 07/05089-9 - Desenvolvimento de nanobiossensores usando a espectroscopia de força atômica: aplicação na detecção de pesticidas
• Beneficiário: Fabio de Lima Leite
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 09/09120-3 - Luciferases de coleópteros: evolução estrutural e funcional, e engenharia para fins biotecnológicos
• Beneficiário: Vadim Viviani
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/04599-6 - Estudo de "superfícies inteligentes" para desenvolvimento de nanobiossensores
• Beneficiário: Aline Cristine Nanuh da Silva
• Linha de fomento: Bolsas no Brasil - Mestrado