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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanobiosensors Based on Chemically Modified AFM Probes: A Useful Tool for Metsulfuron-Methyl Detection

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da Silva, Aline C. N. [1] ; Deda, Daiana K. [1] ; da Roz, Alessandra L. [1] ; Prado, Rogilene A. [2] ; Carvalho, Camila C. [2] ; Viviani, Vadim [2] ; Leite, Fabio L. [1]
Total Authors: 7
[1] Univ Fed Sao Carlos, Dept Phys Chem & Math, Multidisciplinary Lab Nanoneurobiophys, Nanoneurobiophys Res Grp, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Phys Chem & Math, Lab Biochem & Biotechnol Bioluminescence, BR-18052780 Sorocaba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SENSORS; v. 13, n. 2, p. 1477-1489, FEB 2013.
Web of Science Citations: 22

The use of agrochemicals has increased considerably in recent years, and consequently, there has been increased exposure of ecosystems and human populations to these highly toxic compounds. The study and development of methodologies to detect these substances with greater sensitivity has become extremely relevant. This article describes, for the first time, the use of atomic force spectroscopy (AFS) in the detection of enzyme-inhibiting herbicides. A nanobiosensor based on an atomic force microscopy (AFM) tip functionalised with the acetolactate synthase (ALS) enzyme was developed and characterised. The herbicide metsulfuron-methyl, an ALS inhibitor, was successfully detected through the acquisition of force curves using this biosensor. The adhesion force values were considerably higher when the biosensor was used. An increase of similar to 250% was achieved relative to the adhesion force using an unfunctionalised AFM tip. This considerable increase was the result of a specific interaction between the enzyme and the herbicide, which was primarily responsible for the efficiency of the nanobiosensor. These results indicate that this methodology is promising for the detection of herbicides, pesticides, and other environmental contaminants. (AU)

FAPESP's process: 07/05089-9 - Development of nanobiosensors using atomic force spectroscopy: application for detecting pesticides
Grantee:Fabio de Lima Leite
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 10/04599-6 - Development of nanobiosensors based on intelligent surfaces
Grantee:Aline Cristine Nanuh da Silva
Support type: Scholarships in Brazil - Master
FAPESP's process: 09/09120-3 - Beetle luciferases: structural, functional evolution and enegineering for biotechnological purposes
Grantee:Vadim Viviani
Support type: Regular Research Grants