Development of ultra-sensitive methods for RNA detection for the diagnosis of Head and Neck Cancer

• Grant number: 16/16565-5
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): December 01, 2016
• Effective date (End): August 19, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Orlando Fatibello Filho
• Grantee: Fernando Henrique Cincotto
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

This research project has as objective to develop methods for the detection ¼RNA as a biomarker for the diagnosis of Head and Neck Cancer (HNSCC) in real samples. Will be developed and evaluated two methods based on the use of magnetic particles decorated with an RNA probe (PM-sRNA) designed and synthesized for the capture functions, separation and detection of ¼RNA. In one of the methods will be performed the magnetic capturing of the bioconjugate formed by PM-sRNA and µRNA (PM-sRNA-µRNA) directly on the surface of a disposable electrode using a magnetic disc positioned behind the working electrode. In a second method the PM-SENA-¼RNA bioconjugate will be detected using disposable electrode modified with a sequence complementary to the sRNA to capture the bioconjugate. In both cases disposable electrochemical sensors will be developed with new architectures using graphene and semiconductor nanoparticles (quantum dots) for the photoassisted electrochemical detection, from the reduction/oxidation of the quantum dots with high selectivity and low cost. The electrodes will be characterized by measurements of Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) spectroscopy, diffraction X-rays, diffuse reflectance and electrochemical techniques such as cyclic voltammetry and electrochemical impedance spectroscopy. In one of the methods arrangements will be used. The developed methods will be applied in determining ¼RNA in real samples of patients with Head and Neck Cancer that will be provided in partnership with the Barretos Cancer Hospital. It is expected with this project is to develop methods for ¼RNA detection simple, accurate, and cost effective for the early detection of Head and Neck Cancer. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDONCA, CAMILA D.; PRADO, THIAGO M.; CINCOTTO, FERNANDO H.; VERBINNEN, RAPHAEL T.; MACHADO, SERGIO A. S.. Methylparaben quantification via electrochemical sensor based on reduced graphene oxide decorated with ruthenium nanoparticles. SENSORS AND ACTUATORS B-CHEMICAL, v. 251, p. 739-745, NOV 2017. (16/06139-9, 16/16565-5)

PRADO, THIAGO M.; CINCOTTO, FERNANDO H.; MORAES, FERNANDO C.; MACHADO, SERGIO A. S.. Electrochemical Sensor-Based Ruthenium Nanoparticles on Reduced Graphene Oxide for the Simultaneous Determination of Ethinylestradiol and Amoxicillin. Electroanalysis, v. 29, n. 5, p. 1278-1285, MAY 2017. (16/12926-3, 16/16565-5)

SANTOS, ANDERSON MARTIN; WONG, ADEMAR; CINCOTTO, FERNANDO HENRIQUE; MORAES, FERNANDO CRUZ; FATIBELLO-FILHO, ORLANDO. Square-wave adsorptive anodic stripping voltammetric determination of norfloxacin using a glassy carbon electrode modified with carbon black and CdTe quantum dots in a chitosan film. Microchimica Acta, v. 186, n. 3, MAR 2019. (16/12926-3, 16/16565-5, 17/10118-0)

DO PRADO, THIAGO MARTIMIANO; CINCOTTO, FERNANDO HENRIQUE; FATIBELLO-FILHO, ORLANDO; DE MORAES, FERNANDO CRUZ. Bismuth Vanadate/Reduced Graphene Oxide Nanocomposite Electrode for Photoelectrochemical Determination of Diclofenac in Urine. Electroanalysis, v. 30, n. 11, p. 2704-2711, NOV 2018. (16/12926-3, 16/16565-5)

CINCOTTO, FERNANDO H.; FAVA, ELSON L.; MORAES, FERNANDO C.; FATIBELLO-FILHO, ORLANDO; FARIA, RONALDO C.. A new disposable microfluidic electrochemical paper-based device for the simultaneous determination of clinical biomarkers. Talanta, v. 195, p. 62-68, APR 1 2019. (16/10012-4, 16/16565-5, 17/10118-0, 16/12926-3)

FONSECA, WILSON T.; CINCOTTO, FERNANDO H.; LOURENCAO, BRUNA C.; DE ALMEIDA, V, STHEFANE; MORAES, FERNANDO C.; FATIBELLO-FILHO, ORLANDO; DE CARVALHO, ANA C.; CARVALHO, ANDRE L.; MELENDEZ, MATIAS E.; FARIA, RONALDO C.. Ultrasensitive magnetogenoassay for detection of microRNA for diagnosis of metastatic lymph nodes in head and neck cancer using disposable electrodes. SENSORS AND ACTUATORS B-CHEMICAL, v. 352, n. 1, FEB 1 2022. (16/16565-5, 17/24053-7, 16/12926-3, 20/01050-5)

CANEVARI, THIAGO C.; CINCOTTO, FERNANDO H.; GOMES, DELMARCIO; LANDERS, RICHARD; TOMA, HENRIQUE E.. Magnetite Nanoparticles Bonded Carbon Quantum Dots Magnetically Confined onto Screen Printed Carbon Electrodes and their Performance as Electrochemical Sensor for NADH. Electroanalysis, v. 29, n. 8, p. 1968-1975, AUG 2017. (16/16565-5, 16/12519-9, 13/24725-4)

DO PRADO, THIAGO M.; CINCOTTO, FERNANDO H.; MACHADO, SERGIO A. S.. Spectroelectrochemical study of acetylsalicylic acid in neutral medium and its quantification in clinical and environmental samples. Electrochimica Acta, v. 233, p. 105-112, APR 10 2017. (13/17053-0, 16/16565-5, 06/53266-4)

CINCOTTO, FERNANDO H.; CARVALHO, DANIEL A. S.; CANEVARI, THIAGO C.; TOMA, HENRIQUE E.; FATIBELLO-FILHO, ORLANDO; MORAES, FERNANDO C.. A nano-magnetic electrochemical sensor for the determination of mood disorder related substances. RSC ADVANCES, v. 8, n. 25, p. 14040-14047, 2018. (16/12519-9, 16/12926-3, 16/16565-5, 13/24725-4, 11/13312-5)

WONG, ADEMAR; SANTOS, ANDERSON M.; CINCOTTO, FERNANDO H.; MORAES, FERNANDO C.; FATIBELLO-FILHO, ORLANDO; SOTOMAYOR, MARIA D. P. T.. A new electrochemical platform based on low cost nanomaterials for sensitive detection of the amoxicillin antibiotic in different matrices. Talanta, v. 206, JAN 1 2020. (16/16565-5, 14/50945-4, 17/10118-0)