Hypothalamic melanin-concentrating hormone projections to the septo-hippocampal complex in the rat

Autor(es)

Lima, Fernando F. B. ^{[1, 2]} ; Sita, Luciane V. ^[1] ; Oliveira, Amanda R. ^[1] ; Costa, Helder C. ^[1] ; da Silva, Joelcimar M. ^[1] ; Mortara, Renato A. ^[3] ; Haemmerle, Carlos A. S. ^[1] ; Xavier, Gilberto F. ^[4] ; Canteras, Newton S. ^{[5, 6]} ; Bittencourt, Jackson C. ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Chem Neuroanat, BR-05508000 Sao Paulo - Brazil

^[2] Univ Fed Mato Grosso, Sch Med, Dept Basic Hlth Sci, BR-78060900 Cuiaba, Mato Grosso - Brazil

^[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Biosci, Dept Physiol, BR-05508090 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Funct Neuroanat, BR-05508000 Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Psychol, Ctr Neurosci & Behav, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chemical Neuroanatomy; v. 47, p. 1-14, JAN 2013.
• Citações Web of Science: 12

Resumo

Melanin-concentrating hormone (MCH) and neuropeptide glutamic acid-isoleucine (NEI) are expressed in neurons that are located mainly in the hypothalamus and project widely throughout the rat central nervous system. One of the main targets of melanin-concentrating hormone is the hippocampal formation, although the exact origin of the projections is unknown. By using injections of the retrograde tracer True Blue into the hippocampus, together with immunohistochemical analysis, we observed retrogradely labeled melanin-concentrating hormone-containing neurons in the lateral hypothalamic area, incerto-hypothalamic area, perifornical area, the periventricular nucleus of the hypothalamus, and in the internuclear area (between the dorsomedial and ventromedial nuclei of the hypothalamus), as well as a few retrogradely labeled and melanin-concentrating hormone-immunoreactive cells in the supramammillary nucleus. The afferents from the lateral hypothalamic area were confirmed using injection of the anterograde tracer biotinylated dextran amine, which enabled us to use histochemical analysis in order to visualize fibers and terminals in the hippocampal formation. In the medial septal nucleus, we found cholinergic neurons that are also putatively innervated by melanin-concentrating hormone immunoreactive fibers and project to the hippocampal formation. Finally, using two different protocols for immunoperoxidase, we were able to show GABAergic basket cells presumably innervated by melanin-concentrating hormone-immunoreactive fibers in the hippocampal formation. On the basis of the data collected herein, we hypothesize that the MCH/NEI projections from hypothalamic nuclei participate in spatial memory and learning through direct and indirect pathways. These pathways would enable the animal to organize its exploratory behavior during foraging. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 99/03490-0 - Estudo das conexões do núcleo pré-mamilar ventral responsivas a leptin circulante: relação entre o estado nutricional e o comportamento reprodutivo
• Beneficiário: Carol Fuzeti Elias
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/56975-9 - Estudo das aferências imunorreativas ao hormônio concentrador de melanina (MCH) do núcleo accumbens, no rato Long Evans
• Beneficiário: Carlos Alexandre dos Santos Haemmerle
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/13850-3 - MCH e busca alimentar: um estudo envolvendo lesões neuroquímicas seletivas e microinfusão de MCH
• Beneficiário: Luciane Valéria Sita
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 01/13068-5 - Análise comparativa da organização anatômica do peptídeo derivado de leitura alternativa do gene do hormônio concentrador de melanina (MGOP) no sistema nervoso central de roedor e de primata
• Beneficiário: Jackson Cioni Bittencourt
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 00/05530-8 - Módulos multiusuários para suporte de técnicas de biologia celular e molecular do Departamento de Anatomia ICB/USP
• Beneficiário: Jackson Cioni Bittencourt
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 04/13849-5 - Circuitos peptidérgicos envolvidos na organização do comportamento alimentar
• Beneficiário: Jackson Cioni Bittencourt
• Modalidade de apoio: Auxílio à Pesquisa - Temático