DNA repair in Etoposide-induced DNA damage in lymphocytes of breast cancer patients and healthy women

Autor(es)

Teixeira, Ana Claudia ^[1] ; dos Santos, Raquel Alves ^[1] ; Poersch, Aline ^[1] ; Angotti Carrara, Helio Humberto ^[2] ; de Andrade, Jurandyr Moreira ^[2] ; Takahashi, Catarina Satie ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Gynecol & Obstet, BR-14040900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE; v. 2, n. 3, p. 280-288, 2009.
• Citações Web of Science: 9

Resumo

The present study evaluated the basal DNA damage and the cellular response to this damage induced by in vitro administration of Etoposide in lymphocytes donated by twenty untreated breast cancer (BC) patients and twenty age-matched healthy women. Micronucleus (MN) and alkaline Comet assays were performed in cultured peripheral blood lymphocytes (PBL) according to a standard protocol for in vitro treatment with various concentrations of Etoposide or a control. For the Comet Assay, three samples of cells were collected: T-0 (immediately preceding treatment of the cultures), T-1 (immediately after completion of the treatment) and T-2 (four hours after completion of the treatment). MN frequency in the BC group treated with 25 mu M Etoposide (19.1 +/- 7.35) was significantly higher than the control (10.9 +/- 9.87) group. In the alkaline Comet Assay, both the BC group and the healthy women showed the ability to repair Etoposide-induced DNA damage within 4 hours of reincubation. (AU)

• Processo FAPESP: 06/52225-2 - Avaliação da influência dos polimorfismos nos genes XRCC1 e XRCC3 sobre a resposta aos danos induzidos no DNA pelo Etoposideo em pacientes com câncer de mama
• Beneficiário: Ana Claudia Teixeira
• Modalidade de apoio: Bolsas no Brasil - Mestrado