| Texto completo | |
| Autor(es): |
Girotti, Priscila Azevedo
[1]
;
Misawa, Rubia
[1]
;
Palombit, Kelly
[1]
;
Mendes, Cristina Eusebio
[1]
;
Bittencourt, Jackson Cioni
[1]
;
Castelucci, Patricia
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Cell and Tissue Research; v. 353, n. 3, p. 367-380, SEP 2013. |
| Citações Web of Science: | 10 |
| Resumo | |
The colocalization, number, and size of various classes of enteric neurons immunoreactive (IR) for the purinergic P2X(2) and P2X(7) receptors (P2X(2)R, P2X(7)R) were analyzed in the myenteric and submucosal plexuses of control, undernourished, and re-fed rats. Pregnant rats were exposed to undernourishment (protein-deprivation) or fed a control diet, and their offspring comprised the following experimental groups: rats exposed to a normal diet throughout gestation until postnatal day (P)42, rats protein-deprived throughout gestation and until P42, and rats protein-deprived throughout gestation until P21 and then given a normal diet until P42. Immunohistochemistry was performed on the myenteric and submucosal plexuses to evaluate immunoreactivity for P2X(2)R, P2X(7)R, nitric oxide synthase (NOS), choline acetyltransferase (ChAT), calbindin, and calretinin. Double-immunohistochemistry of the myenteric and submucosal plexuses demonstrated that 100% of NOS-IR, calbindin-IR, calretinin-IR, and ChAT-IR neurons in all groups also expressed P2X(2)R and P2X(7)R. Neuronal density increased in the myenteric and submucosal plexuses of undernourished rats compared with controls. The average size (profile area) of some types of neurons in the myenteric and submucosal plexuses was smaller in the undernourished than in the control animals. These changes appeared to be reversible, as animals initially undernourished but then fed a normal diet at P21 (re-feeding) were similar to controls. Thus, P2X(2)R and P2X(7)R are present in NOS-positive inhibitory neurons, calbindin- and calretinin-positive intrinsic primary afferent neurons, cholinergic secretomotor neurons, and vasomotor neurons in rats. Alterations in these neurons during undernourishment are reversible following re-feeding. (AU) | |
| Processo FAPESP: | 04/00746-3 - Análise da distribuição e do código químico dos receptores purinérgicos P2X2, P2X3 e P2X7 do sistema nervoso entérico de animais submetidos a desnutrição protéica pré e pós-natal e a renutrição pós... |
| Beneficiário: | Patricia Castelucci |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |