Skin vasodilation and analgesic effect of a topical nitric oxide-releasing hydrogel

Autor(es)

Vercelino, Rafael ^[1] ; Cunha, Thiago Mattar ^[2] ; Ferreira, Elisa Silva ^[1] ; Cunha, Fernando Q. ^[2] ; Ferreira, Sergio H. ^[2] ; de Oliveira, Marcelo G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Chem, BR-13087970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Dept Pharmacol, Fac Med, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 24, n. 9, p. 2157-2169, SEP 2013.
• Citações Web of Science: 20

Resumo

New approaches based on topical treatments are needed for treating pain and impaired dermal blood flow. We used a topical Pluronic F127 hydrogel containing S-nitrosoglutathione (GSNO) as a prodrug to generate free NO, an effector molecule that exerts both dermal vasodilation and antinociceptive effects. GSNO-containing hydrogels underwent gelation above 12 A degrees C and released free NO at rates that were directly dependent on the GSNO concentration in the range of 50-150 mM. The topical application of this material led to dose-response dermal vasodilation in healthy volunteers and to a reduction of up to 50 % of the hypernociception intensity in Wistar rats that were subjected to inflammatory pain. Mechanistic investigations indicated that the antinociceptive effect of the topical F127/GSNO hydrogels is produced by the local activation of the cGMP/PKG/KATP channel-signaling pathway, which was stimulated by the free NO that diffused through the skin. These results expand the scope of the biomedical applications of this material and may represent a new approach for the topical treatment of inflammatory pain. (AU)

• Processo FAPESP: 09/09800-4 - Efeito de doadores de óxido nítrico na hipernocicepção inflamatória
• Beneficiário: Rafael Vercelino
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/17235-5 - Preparação de formulações de doadores de óxido nítrico e análise de metabólitos
• Beneficiário: Elisa Silva Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica