Expression and localization of N-domain ANG I-converting enzymes in mesangial cells in culture from spontaneously hypertensive rats

Autor(es)

Andrade, Maria Claudina Camargo de ^[1] ; Di Marco, Giovana Seno ; Teixeira, Vicente de Paulo Castro ; Mortara, Renato Arruda ; Sabatini, Regiane Angélica ; Pesquero, João Bosco ; Boim, Miriam Aparecida ; Carmona, Adriana Karaoglanovic ; Schor, Nestor ; Casarini, Dulce Elena ^[10]

Afiliação do(s) autor(es)

^[1] Universidade Federal de São Paulo (UNIFESP). Campus São Paulo - Vila Clementino. Escola Paulista de Medicina - Brasil

^[10] Universidade Federal de São Paulo (UNIFESP). Campus São Paulo - Vila Clementino. Escola Paulista de Medicina - Brasil

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Physiology : Renal Physiology; v. 290, n. 2, p. F364-F375, Feb. 2006.
• Área do conhecimento: Ciências da Saúde - Medicina
• Assunto(s): Hipertensão Células mesangiais Núcleo celular Regulação da expressão gênica

Resumo

The angiotensin-converting enzyme (ACE) profile in urine of hypertensive patients and spontaneously hypertensive rats (SHR; 90- and 65-kDa N-domain ACEs) is different from that of healthy subjects and Wistar rats (190 and 65 kDa). In addition, four ACE isoforms were purified from mesangial cells (MC) of Wistar rats in the intracellular compartment (130 and 68 kDa) and as secreted forms (130 and 60 kDa). We decided to characterize ACE forms from SHR MC in culture. Analysis of the ACE gene showed that SHR MC are able to express ACE mRNA. The concentrated medium and cell homogenate were separately purified by gel filtration and then subjected to lisinopril-Sepharose chromatography. The molecular masses of purified enzymes, 90 kDa for ACEm1A and 65 kDa for ACEm2A (secreted enzymes) and 90 kDa for ACEInth1A and 65 kDa for ACEInth2A (intracellular), were different from those of Wistar MC. The purified enzymes are Cl- dependent, inhibited by enalaprilat and captopril, and able to hydrolyze AcSDKP. Immunofluorescence and cell fractionation followed by Western blotting showed predominant immunoreaction of the 9B9 antiserum for N-domain ACE in the nuclei. The N-domain ACE was localized in the glomerulus from Wistar rats and SHR. ANG II and ANG-(1-7) were localized in the cell cytoplasm and nuclei. The 90-kDa N-domain ACE, described recently as a possible genetic marker of hypertension, was found inside the cell nuclei of SHR MC colocalized with ANG II and ANG-(1-7). The presence of ANG II in the cell nuclei could suggest an important role for this peptide in the transcription of new genes. (AU)

• Processo FAPESP: 03/02575-9 - Estudo molecular e funcional das isoformas N-domínio da enzima conversora de angiotensina I (ECA) de 65 kDa e 90 kDa (possível marcador genético de hipertensão) nas células mesangiais (cm) de ratos Wistar e SHR
• Beneficiário: Maria Claudina Camargo de Andrade
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 02/13290-2 - Isoforma (90 kDa) da enzima conversora de Angiotensina I, potencial marcador genético de hipertensão: processamento, caracterização molecular e funcional, e segregação genética
• Beneficiário: Dulce Elena Casarini
• Modalidade de apoio: Auxílio à Pesquisa - Temático